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A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

10.3K Views

08:43 min

March 10th, 2017

DOI :

10.3791/55199-v

March 10th, 2017


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Fluorescence based Lymphocyte Assay

Chapters in this video

0:05

Title

0:40

Generation of Splenocyte Cell Suspension from Nur77GFP Mouse Spleens

2:07

T Cell Isolation from the Splenocyte Cell Suspension

3:26

T-cell Activation and the Induction of GFP Expression

4:42

High Throughput Screening of Small Molecule

6:05

Results: T and B Cell Receptors Activation Flowcytometry Results and a Representative T Cell HTS of Smal Molecules

7:56

Conclusion

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