Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression
9.3K Views
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10:54 min
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February 10th, 2017
DOI :
February 10th, 2017
•Chapters in this video
0:05
Title
0:59
Isolation of Embryonic Midbrain
2:55
Dissociation and Plating of Cells
5:34
Harvesting Cultured Dopaminergic Neurons for RNA Sequencing
7:04
Generation, Purification and Amplification of First-strand cDNA
9:13
Results: RNA-seq Detection of Expressed Transcripts in Single Dopaminergic Neurons
9:39
Conclusion
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