Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction
50.8K Views
•
08:30 min
•
August 21st, 2016
DOI :
August 21st, 2016
•Palak G. Patel1,2, Shamini Selvarajah1,2, Suzanne Boursalie1,2, Nathan E. How1,2, Joshua Ejdelman3, Karl-Philippe Guerard3, John M. Bartlett4, Jacques Lapointe3, Paul C. Park1, John B. A. Okello1,2, David M. Berman1,2
1Department of Pathology & Molecular Medicine, Queen's University, 2Division of Cancer Biology & Genetics, Queen's Cancer Research Institute, Queen's University, 3Department of Surgery, Division of Urology, McGill University, 4Transformative Pathology Program, Ontario Institute for Cancer Research (OICR)
Chapters in this video
0:05
Title
0:57
Tissue Coring
2:40
Deparaffinization of the FFPE Tissue Cores
3:45
Homogenization of the Deparaffinized Cores
5:07
Digestion with Proteinase K and Nucleotide Separation
6:12
Results: Assessment of RNA and DNA Recovery and Integrity and Representative Performance in Downstream Analysis
7:50
Conclusion
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