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Whole-cell Patch-clamp Recordings from Morphologically- and Neurochemically-identified Hippocampal Interneurons

24.4K Views

14:37 min

September 30th, 2014

DOI :

10.3791/51706-v

September 30th, 2014


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Keywords Whole cell Patch clamp

Chapters in this video

0:05

Title

1:46

Preparation of Acute-hippocampal Slices

3:00

Whole Cell Patch-clamp Recording from Fast-spiking Interneurons

5:58

Extracellular Electrical Stimulation to Evoke GABAB Receptor-mediated Responses

6:56

Paired Recordings of Synaptically Coupled Fast-spiking INs and CA1 PCs

8:42

Visualization and Immunocytochemistry of Fast-spiking Interneurons

11:30

Results: GABAB Receptor-mediated Currents and Presynapitc Inhibition in Identified Hippocampal Parvalbumin Interneurons

13:59

Conclusion

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