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In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

13.1K Views

17:08 min

August 6th, 2014

DOI :

10.3791/51669-v

August 6th, 2014


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Keywords Clonal Tracking

Chapters in this video

0:05

Title

2:28

Mouse Cell Collection, Purification, and Transduction

6:05

Mouse Bone Marrow Transplantation

7:45

Setup for Confocal and Two-photon Microscopy

10:12

Imaging Sternum Bone Marrow Using Confocal and 2-photon Microscopy

12:01

4D Time-lapse Imaging

13:31

Reconstruction and Analysis of 3D Volumes and 4D Time Sequences

14:33

Representative 3D Reconstructions of Sternal Bone Marrow and Other Organs

16:17

Conclusion

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