JoVE Logo

Zaloguj się

Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry

5.0K Views

07:27 min

April 9th, 2021

DOI :

10.3791/62107-v

April 9th, 2021


Przeglądaj więcej filmów

Alkynyl Fatty Acid Analogs

Rozdziały w tym wideo

0:04

Introduction

0:44

Cell Culture

1:25

Preparation and Saponification of Fatty Acid Analogs

3:35

Click Reaction on Cell Lysates

4:12

Click Reaction on Immunoprecipitated Proteins

5:13

Results: Detection of Fatty Acylated Proteins Using Click Chemistry

6:57

Conclusion

Powiązane Filmy

article

10:49

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

12.6K Views

article

11:01

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

9.6K Views

article

08:41

Identification of Fatty Acids in Bacillus cereus

9.5K Views

article

06:44

Hydrolysis of a Ni-Schiff-Base Complex Using Conditions Suitable for Retention of Acid-labile Protecting Groups

9.6K Views

article

09:13

Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve

12.8K Views

article

11:14

Fatty Acid 13C Isotopologue Profiling Provides Insight into Trophic Carbon Transfer and Lipid Metabolism of Invertebrate Consumers

8.0K Views

article

08:31

Detection of Protein S-Acylation using Acyl-Resin Assisted Capture

9.9K Views

article

07:37

Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening

1.9K Views

article

05:44

An Acetyl-Click Chemistry Assay to Measure Histone Acetyltransferase 1 Acetylation

764 Views

article

07:39

Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line

2.8K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone