A Protocol for Immunohistochemistry and RNA In-situ Distribution within Early Drosophila Embryo
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3.6K Views
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14:34 min
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May 6th, 2022
DOI :
May 6th, 2022
•Wei Zhang*1, Xinjuan Lei*1, Xin Zhou*2,3, Boling He1, Liqin Xiao1, Huimin Yue1, Shulin Wang1, Yuting Sun1, Yajun Wu1, Liyang Wang1,4, George Ghartey-Kwansah1, Odell D. Jones5, Joseph L. Bryant6, MengMeng Xu7, Jianjie Ma3, Xuehon Xu1
1National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China/CGDB, Shaanxi Normal University College of Life Sciences, 2Shaanxi Key Laboratory of Ischemic Cardiovascular Disease, Shaanxi Key Laboratory of Brain disorders, Institute of Basic & Translational Medicine, Xi'an Medical University, 3Ohio State University College of Medicine, 4Hematology-Oncology Division, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 5University of Pennsylvania ULAR, 6University of Maryland School of Medicine, 7Columbia University Medical Center
Rozdziały w tym wideo
0:05
Introduction
0:44
Coating Slides
1:41
Embedding of Drosophila Embryos
4:25
Hematoxylin-Eosin Staining
5:25
Periodic Acid-Silver Methenamine Staining
6:28
Immunohistochemistry
8:15
In Situ Hybridization
12:17
Results
13:32
Discussion
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