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Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

27.6K Views

11:22 min

October 15th, 2019

DOI :

10.3791/59980-v

October 15th, 2019


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Microbiota Analysis

Rozdziały w tym wideo

0:04

Title

1:25

Extraction of DNA from Fecal Sample

2:29

16S rRNA Gene Amplification (PCR1) and DNA Library Construction (PCR2)

3:57

Clean-up of Indexed PCR (PCR2) and Quantification

6:03

Sequence Analysis

7:49

Results: Quality Profile and Statistical Analysis

9:29

Conclusion

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