Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity
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12:31 min
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May 1st, 2018
DOI :
May 1st, 2018
•Ganggang Huang*1, Zhenwei Zhong*1, Shane Miersch2,3, Sachdev S. Sidhu1,2,3, Shin-chen Hou1, Donghui Wu1
1Laboratory of Antibody Engineering, Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, 2Banting and Best Department of Medical Research, Terrence Donnelly Center for Cellular and Biomolecular Research, University of Toronto, 3Department of Molecular Genetics, Terrence Donnelly Center for Cellular and Biomolecular Research, University of Toronto
Rozdziały w tym wideo
0:04
Title
1:18
Electroporation and Calculation of the Library Size
6:02
Quality Assessment by Protein A/L Direct Binding ELISA Assay and Sequencing
9:43
Results: Conversion of dU-ssDNA to CCC-DNA and Phage Titration for Calculation of Library Size
11:07
Conclusion
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