Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows
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08:16 min
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January 16th, 2018
DOI :
January 16th, 2018
•Matthew B. O'Rourke1,2, Matthew P. Padula2, Caine Smith3, Priscilla Youssef4, Stuart Cordwell1, Paul Witting4, Greg Sutherland3, Ben Crossett1
1Mass Spectrometry Core Facility, University of Sydney, 2Proteomics Core Facility, University of Technology Sydney, 3Neuropathology Group, Discipline of Pathology, School of Medical Sciences, University of Sydney, 4Redox Biology Group, Discipline of Pathology, School of Medical Sciences, University of Sydney
Rozdziały w tym wideo
0:05
Title
0:29
Preparation of Reagents and Equipment
1:21
Preparation of Tissue Sections and Methylene Crosslink Hydrolysis
2:40
Tissue Digestion
3:57
Matrix Coating via Sublimation
5:26
Recrystallization and Instrumentation
6:20
Results: Correctly and Incorrectly Prepared Human Brain Tissue
7:07
Conclusion
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