Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
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10:52 min
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September 28th, 2017
DOI :
September 28th, 2017
•Robert Warneford-Thomson1,4, Chongsheng He1,2, Simone Sidoli1,3, Benjamin A. Garcia1,3, Roberto Bonasio1,2
1Epigenetics Institute, University of Pennsylvania Perelman School of Medicine, 2Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, 3Department of Biochemistry and Biophysics, University of Pennsylvania Perelman School of Medicine, 4Graduate Group in Biochemistry and Biophysics, University of Pennsylvania Perelman School of Medicine
Rozdziały w tym wideo
0:05
Title
1:04
Crosslink of Protein-RNA Interactions in Live Cells
3:26
Isolation of Nuclei
4:27
Lysis of Nuclei and Trypsin Digestion
5:51
Desalting of Peptides
7:39
Removal of Crosslinked RNA
8:44
Results: RNA-binding Protein and RNA-binding Region Identification
10:04
Conclusion
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