A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry
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08:46 min
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July 12th, 2017
DOI :
July 12th, 2017
•Ana C. Lima*1,2,3,4, Min Jung*1, Jannette Rusch1, Abul Usmani1, Alexandra M. Lopes3,4, Donald F. Conrad1
1Department of Genetics, Washington University School of Medicine, 2Graduate Program in Areas of Basic and Applied Biology (GABBA), Abel Salazar Institute of Biomedical Sciences, University of Porto, 3Instituto de Investigação e Inovação em Saúde, University of Porto, 4IPATIMUP - Instituto de Patologia e Imunologia Molecular da Universidade do Porto, 5Department of Pathology & Immunology, Washington University School of Medicine
Rozdziały w tym wideo
0:05
Title
1:10
Testicular Tissue Single Cell Suspension Generation
2:26
Cell Staining and Fluorescence Activiated Cell Sorting (FACS)
5:33
Results: Representative Testicular Cell Subset Analyses
7:17
Conclusion
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