TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis
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13:47 min
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January 29th, 2015
DOI :
January 29th, 2015
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Rozdziały w tym wideo
0:05
Title
3:32
Cell Imaging by Total Internal Reflection Fluorescence Microscopy (TIRFM)
6:08
Fluorescence Intensity Quantification
7:09
Photobleaching Correction and Threshold Determination
8:16
Selection of Fusion Events Using a Semiautomatic Procedure
10:01
Data Analysis
11:31
Results: Analysis of Synaptic Vesicles Fusion Under Resting and Stimulated (Potassium-induced Depolarization) Conditions Are Shown
12:37
Conclusion
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