소에 이펙터 및 메모리 T 세포 반응을 평가하기위한 장기 배양 인터페론-γ 효소 링크 된 Immunospot 분석의 응용 프로그램

1:40

Methods

2:05

Long-term Culture

3:38

Elispot Assay Day 1

4:30

Elispot Assay Day 2

5:15

Adherent Cell Isolation

5:37

Flow Cytometry Plate Preparation

6:15

Harvest the 13 Day Long-term Cells

9:05

Elispot Assay Day 3

10:04

Alkaline Phosphatase Preparation

11:08

Substrate Solution Preparation

11:55

Reading the Plate

12:12

Performing Flow Cytometry

12:25

Results

14:37

Conclusions