Name: morris water maze test: optimization for mouse strain and testing environment
Uploaded: 2015-06-22T13:00:00.000+00:00
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Description: Watch this Scientific Journal Video about Morris Water Maze Test: Optimization for Mouse Strain and Testing Environment at JoVE.com

신경 질환 및 뇌졸중의 국립 연구소, -이 작품은 일반 의료 과학 국립 연구소 (U54GM104942 리드 / 잉글 러 - Chiurazzi)에 의해 지원되었다 (애쉬 - R01NS33249, R01NS63249 및 R01NS79374), 코 브레 (잉글 러 - Chiurazzi - P20GM109098) 알츠하이머 협회 (리드 - NIRG-12-242187), WVU 교수 연구 상원 그랜트 (리드), 의학 딘의 사무실의 WVU 대학 (잉글 러 - Chiurazzi)에서 WVU PSCOR 보조금 (리드) 및 내부 자금. 내용은 전적으로 저자의 책임이며 반드시 NIH 또는 알츠하이머 협회의 공식 견해를 대변하지 않습니다.

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저자가 공개하는 게 없다.

MWM 태스크 널리 공간 학습 및 기억을 평가하는 데 사용된다. 그러나,이 작업의 안정성은 많은 요인들에 의해 영향을 배경 균주 및 테스트 환경 모두의 최적화를 필요로 할 수있다. 도 4는 동일한 트레이닝 프로토콜에 나타낸 두 개의 다른 테스트 실 (동등한 크기 및 레이아웃)에 사용되는 시각적 적용된 상당히 상이한 프로브의 성능을 수득 하였다. 테스트 룸의 많은 기능이 공간 큐 <su...

형질 전환 마우스 모델은 알츠하이머 병 (AD)의 병태 생리학뿐만 아니라, 치료 적 개입의 가능성을 평가하는 수단이되어왔다. 이러한 모리스 물 미로 (MWM)인지 적 작업은 일반적으로 기억력 결핍의 분자 상관 관계를 파악하고 전임상 약물의 효능을 평가하기 위해 이러한 모델과 함께 사용된다. 이는인지 작업의 동적 범위는 치료 효과 미묘한 검출 충분히 넓은 것이 그러나 중요하다. 광고의 마우스 모델,인지 적자는 일반적으로 나이에 따라 달라집니다, 그리고 마우스는 성능이 점진적 감소를 표시 (예를 들면, 1). 민감한 태스크인지를 사용하여 동물 노화와 관련된 비용을 줄일 이전 동물의 생명에 미묘한 차이를 검출 할 수있다. 예를 들면, 해마 의존적 반스 훈련 실험의 수를 줄이는 것이 15-5 미로 D 결과, 작업의 어려움을 증가보다 이른 나이에 3xTg 모델에서 적자의 금 속 탐은 이전에 2를보고했다. 적자 이전 검출뿐만 아니라 이것은 또한인지 결핍의 기초 분자의 변화를 식별 할 수있는 가능성이 높아, 상당한 시간과 비용 절감을 제공한다. 인지 작업의 감도에 영향을 미치는 하나의 요인은 마우스 모델의 유전 배경 균주이다. 예를 들어, BALB / c 마우스는 C57BL / 6 (3)과 같은 다른 균주에 비해 과제 학습 및 기억에 우수한 성능을 나타낸다. F1 FVB / N X 129S6 배경은 AD의 가장 널리 사용되는 모델 Tg2576 및 RTG (TauP301L) 4510 모델이 사용된다. 이 균주는 B6 / SJL 마우스 (4)를 포함하여 다른 균주에 MWM 상대적으로 우수한 학습 능력을 나타낸다. 이 때문에 우수한 학습 능력, 광범위한 훈련 후 단일 프로브의 사용은 오버 - 트레이닝으로 인한 그룹 차이를 마스크 할 수있다. 또한, sensitivit프로브 시험의 Y는 연령에 따라 달라질 수 있습니다. 우리는 이전에 이전 프로브 시험은 제한 숨겨진 플랫폼 훈련 후,보다 젊은 형질 전환 유전자 음성 한배의 새끼 컨트롤에 비해 젊은 Tg2576의 차이에 더 민감 더 광범위한 훈련 (5) 다음에 삽입 프로브 시험 있음을 보여 주었다. 이전 프로브 시험 5보다 대조적으로, 광범위한 교육을 다음과 같은 검사 시험은 이전 한배 새끼에 비해 Tg2576 마우스 이전 (20 ~ 25 월)에 더 민감하다. 길이 시험이 수행되고, 특정 프로브 시험의 감도 연령 의존적 인 경우 특히 훈련 ​​내내 프로브 시험을 산재하여 민감한 재판이 식별 될 가능성이 증가된다. 1 F1 FVB / N (X)의 우수한 성능을 보여준다 더 광범위한 훈련 프로토콜에서 훈련 B6 / SJL 배경의 마우스에 비해이 변형에 최적화 된 프로토콜에 따라 129S6 마우스. MWM은일반적으로 시간과 실험실 (6) 모두에서 재현 할 수있는 신뢰할 수있는 수단을 제공하기 위해 생각했다. 예를 들어, 원래 우리의 미네소타 실험실 1,7에서 사용되는 기본 프로토콜이 성공적으로 웨스트 버지니아 대학 8에 약간의 수정으로 구현되었습니다. 마찬가지로, 손상 동등한 수준 무균 또는 통상 조건 하에서 구 수납 경우 한배 새끼를 제어하기 위해 4510 생쥐 상대 RTG (TauP301L)에서 관찰되었다. 그러나, 테스트 환경 MWM 태스크의 민감도에 영향을 미칠 수있다. 같은 실내 조명, 환기구, 온도 구배 및 소음 등의 모든 요소가 궁극적으로 성능에 영향을 미칠 수있는 환경 단서 4에 기여한다. 우리 미네소타 실험실 동물 사육장은 신축 이동 하였을 때, 최대 야생형 성능의 38 % 감소는 실질적으로 작업 및 트랜스 - 관련 결핍을 탐지 할 수있는 능력의 동적 범위를 감소가 관찰되었다. PE의 변화rformance 동등한 크기 및 구성으로 검사실을 설계하고, 동일한인가 시각적 큐를 사용에도 불구하고 발생 하였다. 본래의 프로토콜 A &quot;재 최적화가&quot;새로운 테스트 환경에서 MWM 태스크의 동적 범위를 증가시키기 위해 요구되었다. 여기에 F1 FVB / N와 함께 사용하기위한 맞춤형 원래 프로토콜은 129S6 배경 5 설명 X. 일부 연구는 응력을 제시하기 때문에 성능 (11)이 스트레스 유도 결손 완화 불량한 MWM 성능 (10) 및 사전 처리와 연결되어, 사전 처리 프로토콜은 풀의 도입 및 제거 전에 MWM 테스트 행에 쥐를 순응하도록 설계되었다 . 사전 처리 후, 마우스 제기 플랫폼 플래그가 표시되는 볼 플랫폼 교육을 받다. 눈에 보이는 플랫폼 훈련은 감각 이상과 관련된 성능 문제로 쥐를 식별하는 데 사용됩니다. 제자에 설명 된 배제 기준을 사용하여ocol 섹션, 성능 무능한 마우스 숨겨진 플랫폼 교육 및 프로브 시험 이후의 시험에서 제거됩니다. 감각 성능 데이터에서 고려되기 때문에 숨겨진 플랫폼 교육 및 프로브 시험에서 장애인지 적자로 해석됩니다. 가시 플랫폼 훈련 종료 후, 마우스는 플랫폼을 물에 잠긴 외부 큐에 대해 동일한 위치에 유지된다 숨겨진 플랫폼 트레이닝을 시작. 플랫폼 (프로브 시험)을 제거하는 시험이 추가 교육의 영향을 평가하기 위해 숨겨진 플랫폼 교육 전반에 걸쳐 산재되어있다. 프로브 실험은 하루의 시작 부분에서 발생하기 때문에 추가적인 숨겨진 플랫폼 트레이닝 전에, 프로브 실험은 20 시간 지연에 따라 플랫폼의 위치를 기억하는 동물의 능력을 측정하고, 기준 메모리 (12)의 측정 값을 고려 하였다. 마지막으로, 방법은이 프로토콜은 원래 다시 최적화 될 때 테스트 환경 변화중단 제어 성능이 설명되어 있습니다.

<table><tbody><tr><td>Viewer Tracking software</td><td>Biobserve</td><td></td><td>This particular software is not a requirement - there are other tracking systems available</td></tr><tr><td>Pre-handling pool</td><td></td><td></td><td>Dimensions approximately 1 foot wide x 2 feet long x 1.5 feet deep</td></tr><tr><td>Plastic beaker</td><td></td><td></td><td>1 L</td></tr><tr><td>Scoop</td><td></td><td></td><td></td></tr><tr><td>Small net</td><td></td><td></td><td></td></tr><tr><td>Stopwatch</td><td></td><td></td><td></td></tr><tr><td>Non-toxic white tempera paint</td><td></td><td></td><td>Any color paint can be used; however, most tracking software programs require that the paint contrast with the color of the animal.</td></tr><tr><td>Visible platform</td><td></td><td></td><td>Color should contrast that of maze</td></tr><tr><td>Curtain rod</td><td></td><td></td><td></td></tr><tr><td>Curtains</td><td></td><td></td><td></td></tr><tr><td>Circular tub</td><td></td><td></td><td>Usually white in color; approximately 4 feet in diameter</td></tr><tr><td>Hidden platform</td><td></td><td></td><td>Painted same color as the water</td></tr></tbody></table>

morris water maze test: optimization for mouse strain and testing environment

The Morris water maze (MWM) is a commonly used task to assess hippocampal-dependent spatial learning and memory in transgenic mouse models of disease, including neurocognitive disorders such as Alzheimer&#8217;s disease. However, the background strain of the mouse model used can have a substantial effect on the observed behavioral phenotype, with some strains exhibiting superior learning ability relative to others. To ensure differences between transgene negative and transgene positive mice can be detected, identification of a training procedure sensitive to the background strain is essential. Failure to tailor the MWM protocol to the background strain of the mouse model may lead to under- or over- training, thereby masking group differences in probe trials. Here, a MWM protocol tailored for use with the F1 FVB/N x 129S6 background is described. This is a frequently used background strain to study the age-dependent effects of mutant P301L tau (rTg(TauP301L)4510 mice) on the memory deficits associated with Alzheimer&#8217;s disease. Also described is a strategy to re-optimize, as dictated by the particular testing environment utilized.

우리는 그림 3은 베타 - 아밀로이드 (Tg2576 마우스) 및 돌연변이 P301L 타우 공간 참조 메모리 (RTG (TauP301L) 4510 마우스) (예를 들면, 1,5,7,8).의 효과를 연구하기 위해 모리스 물 미로를 사용했다 대표적인 결과는 모터 및 시각적 능력을 평가하기 위해 테스트 환경 A.을 이용하는, 학습 및 기억 8 성인 발병 P301L 타우 발현의 효과를 조사 연구에서보고 된 마우스는 각 트?...

Watch this Scientific Journal Video about Morris Water Maze Test: Optimization for Mouse Strain and Testing Environment at JoVE.com

Morris Water Maze Test: Optimization for Mouse Strain and Testing Environment

This manuscript describes a Morris water maze (MWM) protocol tailored for use with a commonly used mouse model of Alzheimer's disease. The MWM is widely used in transgenic mouse models. Implementation of a procedure sensitive to the background strain of the mouse model is essential for detecting group differences.

모리스 물 미로 테스트 : 마우스 스트레인 및 테스트 환경을위한 최적화

 The Morris water maze (MWM) is a commonly used task to assess hippocampal-dependent spatial learning and memory in transgenic mouse models of disease, ...

morris-water-maze-test-optimization-for-mouse-strain-testing

Universidad San Sebastian

Research

JoVE Journal

Behavior

21.4K 조회수.  West Virginia University.  This manuscript describes a Morris water maze (MWM) protocol tailored for use with a commonly used mouse model of Alzheimer's disease. The MWM is widely used in transgenic mouse models. Implementation of a procedure sensitive to the background strain of the mouse model is essential for detecting group differences. 

비디오: Morris Water Maze Test: Optimization for Mouse Strain and Testing Environment

Shallow Water (Paddling) Variants of Water Maze Tests in Mice

When Richard Morris devised his water maze in 19817, most behavioral work was done in rats. However, the greater understanding of mouse genetics led to the mouse becoming increasingly important. But researchers found that some strains of mutant mice were prone to problems like passively floating or diving when they were tested in the Morris water maze11. This was unsurprising considering their natural habitat; rats swim naturally (classically, the "sewer rat"), whereas mice evolved in the dry areas of central Asia.
To overcome these problems, it was considered whether shallow water would be a sufficient stimulus to provide escape motivation for mice. This would also avoid the problems of drying the small creatures with a towel and then putting them in a heated recovery chamber to avoid hypothermia, which is a much more serious problem than with rats; the large ratio of surface area to volume of a mouse makes it particularly vulnerable to rapid heat loss.
Another consideration was whether a more natural escape strategy could be used, to facilitate learning. Since animals that fall into water and swim away from the safety of the shore are unlikely to pass on their genes, animals have evolved a natural tendency to swim to the edge of a body of water. The Morris water maze, however, requires them to swim to a hidden platform towards the center of the maze - exactly opposite to their evolved behavior. Therefore the paddling maze should incorporate escape to the edge of the apparatus. This feature, coupled with the use of relatively non-aversive shallow water, embodies the "Refinement" aspect of the "3 Rs" of Russell and Burch8.
Various types of maze design were tried; the common feature was that the water was always shallow (2 cm deep) and escape was via a tube piercing the transparent wall of the apparatus. Other tubes ("false exits") were also placed around the walls but these were blocked off. From the inside of the maze all false exits and the single true exit looked the same. Currently a dodecagonal (12-sided) maze is in use in Oxford, with 12 true/false exits set in the corners. In a recent development a transparent paddling Y-maze has been tested successfully.

Shallow Water Paddling Variants of Water Maze Tests in Mice

Mice can swim, but many strains appear to find this activity stressful. To overcome this problem mazes have been devised where escape from shallow water is used to motivate behaviour. These have been demonstrated to support learning at least as good as the traditional and widely used Morris water maze.

마우스의 물 미로 시험의 얕은 물 (패들링) 변종

When Richard Morris devised his water maze in 19817, most behavioral work was done in rats. However, the greater understanding of mouse genetics led to ...

연구

행동분석학

Low-stress Route Learning Using the Lashley III Maze in Mice

Many behavior tests designed to assess learning and memory in rodents, particularly mice, rely on visual cues, food and/or water deprivation, or other aversive stimuli to motivate task acquisition. As animals age, sensory modalities deteriorate. For example, many strains of mice develop hearing deficits or cataracts. Changes in the sensory systems required to guide mice during task acquisition present potential confounds in interpreting learning changes in aging animals. Moreover, the use of aversive stimuli to motivate animals to learn tasks is potentially confounding when comparing mice with differential sensitivities to stress. To minimize these types of confounding effects, we have implemented a modified version of the Lashley III maze. This maze relies on route learning, whereby mice learn to navigate a maze via repeated exposure under low stress conditions, e.g. dark phase, no food/water deprivation, until they navigate a path from the start location to a pseudo-home cage with 0 or 1 error(s) on two consecutive trials. We classify this as a low-stress behavior test because it does not rely on aversive stimuli to encourage exploration of the maze and learning of the task. The apparatus consists of a modular start box, a 4-arm maze body, and a goal box. At the end of the goal box is a pseudo-home cage that contains bedding similar to that found in the animal&#x2019;s home cage and is specific to each animal for the duration of maze testing. It has been demonstrated previously that this pseudo-home cage provides sufficient reward to motivate mice to learn to navigate the maze1. Here, we present the visualization of the Lashley III maze procedure in the context of evaluating age-related differences in learning and memory in mice along with a comparison of learning behavior in two different background strains of mice. We hope that other investigators interested in evaluating the effects of aging or stress vulnerability in mice will consider this maze an attractive alternative to behavioral tests that involve more stressful learning tasks and/or visual cues.

The Lashley III maze is a route-learning task that does not rely on aversive stimuli or visual cues. It is thus a highly attractive option for evaluating learning and memory, especially in aging mice or otherwise where stress is a consideration.

마우스에 레쉴리 III 메이즈를 사용하여 낮은 스트레스 루트 학습

Many behavior tests designed to assess learning and memory in rodents, particularly mice, rely on visual cues, food and/or water deprivation, or other ...

신경과학

Quantifying Cognitive Decrements Caused by Cranial Radiotherapy

With the exception of survival, cognitive impairment stemming from the clinical management of cancer is a major factor dictating therapeutic outcome. For many patients afflicted with CNS and non-CNS malignancies, radiotherapy and chemotherapy offer the best options for disease control. These treatments however come at a cost, and nearly all cancer survivors (~11 million in the US alone as of 2006) incur some risk for developing cognitive dysfunction, with the most severe cases found in patients subjected to cranial radiotherapy (~200,000/yr) for the control of primary and metastatic brain tumors1. Particularly problematic are pediatric cases, whose long-term survival plagued with marked cognitive decrements results in significant socioeconomic burdens2. To date, there are still no satisfactory solutions to this significant clinical problem.
We have addressed this serious health concern using transplanted stem cells to combat radiation-induced cognitive decline in athymic rats subjected to cranial irradiation3. Details of the stereotaxic irradiation and the in vitro culturing and transplantation of human neural stem cells (hNSCs) can be found in our companion paper (Acharya et al., JoVE reference). Following irradiation and transplantation surgery, rats are then assessed for changes in cognition, grafted cell survival and expression of differentiation-specific markers 1 and 4-months after irradiation. To critically evaluate the success or failure of any potential intervention designed to ameliorate radiation-induced cognitive sequelae, a rigorous series of quantitative cognitive tasks must be performed. To accomplish this, we subject our animals to a suite of cognitive testing paradigms including novel place recognition, water maze, elevated plus maze and fear conditioning, in order to quantify hippocampal and non-hippocampal learning and memory. We have demonstrated the utility of these tests for quantifying specific types of cognitive decrements in irradiated animals, and used them to show that animals engrafted with hNSCs exhibit significant improvements in cognitive function3.
The cognitive benefits derived from engrafted human stem cells suggest that similar strategies may one day provide much needed clinical recourse to cancer survivors suffering from impaired cognition. Accordingly, we have provided written and visual documentation of the critical steps used in our cognitive testing paradigms to facilitate the translation of our promising results into the clinic.

Cognitive impairment resulting from the radiotherapeutic management of brain tumors represents a clinically intractable condition that adversely impacts quality of life. The capability to critically evaluate potential interventions for ameliorating radiation-induced cognitive decrements ultimately depends on the capability to undertake rigorous quantitative assessments of cognition.

두개골 방사선 치료에 의한인지 감소를 Quantifying

With the exception of survival, cognitive impairment stemming from the clinical management of cancer is a major factor dictating therapeutic outcome. For ...

의학

Morris Water Maze Test for Learning and Memory Deficits in Alzheimer&#39;s Disease Model Mice

The Morris Water Maze (MWM) was first established by neuroscientist Richard G. Morris in 1981 in order to test hippocampal-dependent learning, including acquisition of spatial memoryand long-term spatial memory 1. The MWM is a relatively simple procedure typically consisting of six day trials, the main advantage being the differentiation between the spatial (hidden-platform) and non-spatial (visible platform) conditions 2-4. In addition, the MWM testing environment reduces odor trail interference 5. This has led the task to be used extensively in the study of the neurobiology and neuropharmacology of spatial learning and memory. The MWM plays an important role in the validation of rodent models for neurocognitive disorders such as Alzheimer&#x2019;s Disease 6, 7. In this protocol we discussed the typical procedure of MWM for testing learning and memory and data analysis commonly used in Alzheimer&#x2019;s disease transgenic model mice.

Morris Water Maze Test for Learning and Memory Deficits in Alzheimer's Disease Model Mice

The Morris Water Maze is a behavioral task to test hippocampal-dependent learning and memory. It has been widely used in the study of neurobiology, neuropharmacology and neurocognitive disorders in rodent models.

알츠하이머병 모델 마우스에서 학습 및 메모리 적자에 대한 모리스 워터 메이즈 테스트

The Morris Water Maze (MWM) was first established by neuroscientist Richard G. Morris in 1981 in order to test hippocampal-dependent learning, including ...

T-maze Forced Alternation and Left-right Discrimination Tasks for Assessing Working and Reference Memory in Mice

Forced alternation and left-right discrimination tasks using the T-maze have been widely used to assess working and reference memory, respectively, in rodents. In our laboratory, we evaluated the two types of memory in more than 30 strains of genetically engineered mice using the automated version of this apparatus. Here, we present the modified T-maze apparatus operated by a computer with a video-tracking system and our protocols in a movie format. The T-maze apparatus consists of runways partitioned off by sliding doors that can automatically open downward, each with a start box, a T-shaped alley, two boxes with automatic pellet dispensers at one side of the box, and two L-shaped alleys. Each L-shaped alley is connected to the start box so that mice can return to the start box, which excludes the effects of experimenter handling on mouse behavior. This apparatus also has an advantage that in vivo microdialysis, in vivo electrophysiology, and optogenetics techniques can be performed during T-maze performance because the doors are designed to go down into the floor. In this movie article, we describe T-maze tasks using the automated apparatus and the T-maze performance of &alpha;-CaMKII+/- mice, which are reported to show working memory deficits in the eight-arm radial maze task. Our data indicated that &alpha;-CaMKII+/- mice showed a working memory deficit, but no impairment of reference memory, and are consistent with previous findings using the eight-arm radial maze task, which supports the validity of our protocol. In addition, our data indicate that mutants tended to exhibit reversal learning deficits, suggesting that &alpha;-CaMKII deficiency causes reduced behavioral flexibility. Thus, the T-maze test using the modified automatic apparatus is useful for assessing working and reference memory and behavioral flexibility in mice.

This article presents the protocol of T-maze tests using a modified automated apparatus for assessing the learning and memory functions in mice.

T-미로 강제 교대 및 근무와 마우스의 레퍼런스 메모리를 평가하기위한 왼쪽 오른쪽 차별 작업

Forced alternation and left-right discrimination tasks using the T-maze have been widely used to assess working and reference memory, respectively, in ...

Morris Water Maze Experiment

The Morris water maze is widely used to study spatial memory and learning. Animals are placed in a pool of water that is colored opaque with powdered non-fat milk or non-toxic tempera paint, where they must swim to a hidden escape platform. Because they are in opaque water, the animals cannot see the platform, and cannot rely on scent to find the escape route. Instead, they must rely on external/extra-maze cues. As the animals become more familiar with the task, they are able to find the platform more quickly. Developed by Richard G. Morris in 1984, this paradigm has become one of the "gold standards" of behavioral neuroscience.

The Morris water maze is a well-accepted tool used to document the involvement of the hippocampus in a behavioral task.

모리스 워터 메이즈 실험

The Morris water maze is widely used to study spatial memory and learning. Animals are placed in a pool of water that is colored opaque with powdered ...

생물학

A Behavioral Test Battery for the Repeated Assessment of Motor Skills, Mood, and Cognition in Mice

Pharmacological and toxicological studies in neurodegeneration require comprehensive behavioral analysis in mice because motor dysfunctions and dysfunctions in mood and cognition are common and often shared symptoms in neurodegenerative diseases. Shown here is a behavioral test battery for motor, mood, and cognition, which can be repeatedly tested in a longitudinal study. This battery assesses the overall behavioral phenotype in mice by examining each domain of behavior with at least two independent well-accepted tests (i.e., open-field test and rotarod test for motor function, social interaction test, elevated plus maze test, and forced swim test for emotional function, and Morris water maze test and novel object recognition test for cognitive function). Therefore, this sensitive and comprehensive test battery is a powerful tool for the study of behavioral alternation in neurodegeneration.

A comprehensive behavioral test battery of motor skills, mood&#8212;including social interaction, depression, and anxiety&#8212;and cognition is designed for the repeated assessment of neurodegeneration-related behavioral changes in mice.

모터 기술, 분위기, 그리고 쥐에 있는 인식의 반복 된 평가 행동 테스트 배터리

Pharmacological and toxicological studies in neurodegeneration require comprehensive behavioral analysis in mice because motor dysfunctions and ...

Analysis of Learning and Memory Ability in an Alzheimer's Disease Mouse Model using the Morris Water Maze

A Morris water maze (MWM) experiment forces experimental animals to swim and learn to find a platform hidden in the water. It is widely used in scientific research to assess the learning and memory of animals. Due to the extensive use of the MWM test, visual experimental protocols are essential for researchers. This manuscript uses the latest studies to introduce the protocol of the MWM test. Alzheimer&#8217; Disease (AD) is characterized by a progressive loss of memory and cognitive function. An alternative and complementary treatment used for AD is Manual Acupuncture (MA). To assess the learning and memory ability of AD model mice, the MWM test was conducted. The visible platform trial, hidden platform trial, probe trial, and reversal trial of MWM were used to evaluate spatial learning and memory ability. In the visible platform trial, the swimming speed and escape latency of mice in different groups was not significantly different. In the hidden platform and reversal trials, the AD group showed a long escape latency. The escape latency decreased significantly after the MA treatment. Low platform crossover number and the proportion of time in the SW quadrant in the probe trial increased after the MA treatment (p &#60; 0.05 or p &#60; 0.01). The results of the MWM tests suggest that MA can effectively improve the spatial learning and memory abilities of AD model mice. Rigorous experimental operations provided assurance of the reliability of the results.

Herein, a protocol to conduct the Morris water maze tests to evaluate the ability of learning and memory of Alzheimer&#8217;s Disease model mice and to assess the effect of manual acupuncture for treating them is described.

모리스 워터 미로를 이용한 알츠하이머 병 마우스 모델의 학습 및 기억 능력 분석

A Morris water maze (MWM) experiment forces experimental animals to swim and learn to find a platform hidden in the water. It is widely used in scientific ...

침술을 이용한 만성 저산소증 마우스의 인지 개선

Acupuncture Treatment in a Mouse Model of Chronic Hypoxia-Induced Cognitive Dysfunction

The treatment of central nervous disorders has consistently posed significant challenges to the medical field. Acupuncture, a non-pharmacological practice rooted in traditional Chinese medicine, entails the insertion of fine needles into precise points on the body and is commonly employed for the management of diverse conditions. Recently, acupuncture has emerged as a promising therapeutic intervention for a range of neurological diseases, including anxiety and respiratory disorders. However, the potential of acupuncture in treating cognitive dysfunction induced by chronic hypoxia has not yet been explored. This paper presents a comprehensive protocol for establishing a mouse model of chronic hypoxia-induced cognitive impairment, administering mild anesthesia, performing acupuncture treatment, and assessing behavioral changes and memory abilities using open field tests and water mazes. The step-by-step protocol provides detailed instructions on accurately locating and positioning acupoints and needles for cognitive improvement. By employing this protocol, researchers can conduct systematic studies to thoroughly evaluate the therapeutic potential of acupuncture for cognitive dysfunction.

저자 스포트라이트: Improving Anesthesia Protocols for Enhanced Mouse Acupuncture Research (향상된 마우스 침술 연구를 위한 마취 프로토콜 개선)

Here, we describe a protocol for implementing mild anesthesia and acupuncture treatment on a chronic hypoxia mouse model and conducting behavioral tests to assess the cognitive alterations post-treatment.

만성 저산소증 유발 인지 기능 장애의 마우스 모델에서 침술 치료

The treatment of central nervous disorders has consistently posed significant challenges to the medical field. Acupuncture, a non-pharmacological practice ...

모리스 물 미로 테스트 : 마우스 스트레인 및 테스트 환경을위한 최적화

요약

더 많은 비디오 탐색

이 비디오의 챕터

마우스의 물 미로 시험의 얕은 물 (패들링) 변종

마우스에 레쉴리 III 메이즈를 사용하여 낮은 스트레스 루트 학습

두개골 방사선 치료에 의한인지 감소를 Quantifying

알츠하이머병 모델 마우스에서 학습 및 메모리 적자에 대한 모리스 워터 메이즈 테스트

T-미로 강제 교대 및 근무와 마우스의 레퍼런스 메모리를 평가하기위한 왼쪽 오른쪽 차별 작업

모리스 워터 메이즈 실험

모터 기술, 분위기, 그리고 쥐에 있는 인식의 반복 된 평가 행동 테스트 배터리

모리스 워터 미로를 이용한 알츠하이머 병 마우스 모델의 학습 및 기억 능력 분석

만성 저산소증 유발 인지 기능 장애의 마우스 모델에서 침술 치료

만성 저산소증 유발 인지 기능 장애의 마우스 모델에서 침술 치료