Scansione retrovirale: Mappatura dei siti di integrazione MLV per definire regioni regolatori specifiche per le cellule

0:45

MLV Transduction of Human Cells

1:41

Restriction Enzyme Digestion and Linker Ligation

3:05

First-round Linker-mediated PCR

3:43

Second Round Linker-mediated PCR

5:17

Library Preparation and Dilution for Massive Sequencing of MLV Integration Sites

7:53

Results: Mapping MLV Integration into Epigenetically-defined Regulatory Regions

9:23

Conclusion