Name: the rodent psychomotor vigilance test (rpvt): a method for assessing neurobehavioral performance in rats and mice
Uploaded: 2016-12-29T12:00:00.000+00:00
Duration: 7 min 47 s
Description: Watch this Scientific Journal Video about The Rodent Psychomotor Vigilance Test rPVT: A Method for Assessing Neurobehavioral Performance in Rats and Mice at JoVE.com

This research was supported by NASA (NNX15AC17G to CMD), and by NASA cooperative agreement NCC 9-58 (E000010 to CMD, NBPF02802 and NBPF04201 to RDH) with the National Space Biomedical Research Institute.

根据公共卫生服务（PHS）政策上的人文关怀和使用实验动物实验动物护理了。所有程序均严格按照该指南中的建议，美国国立卫生研究院的实验动物的护理和使用进行。约翰·霍普金斯大学的机构动物护理和使用委员会批准的方案和所有程序。约翰霍普金斯大学也是由协会实验动物的评估和认可委员会（AAALAC）维护其计划的认可。 1.动物<ol><li>使用成年雄性龙Evans大鼠（大约10 - 12周在行为训练开始老），尽管其它菌株是适用的，包括近交系大鼠。 </li><li>一旦在殖民地的到来，房屋老鼠单独核定笼养和客房，由于粮食需求的限制如下所述。驯化动物住房情况以及处理的多个DAYS之前开始限食过程（约7天）。 </li><li>提供1鼠食自由采食 - 2周让老鼠实现了"自由喂养"的重量。一旦在这个重量，限制食物进入减少大鼠的重量，在1 - 2周，85 - 自由摄食重量的90％。行为训练和测试过程中他们的自由喂养的权重为90％ - 保持在85的动物。先从15 - 17 3g的一天的食物和调整基于速率是否维持其目标重量这一数额。 注意:食物相通常需要一个人的体制ACUC或启动限制之前，其他监管机构审批。 </li><li>每天称量动物并记录体重表的重量。确保称量在相同时间和在相同的规模之前的任何行为训练或馈送开始每天进行。 注:只有启动训练前（下）当动物有实现了他们的目标权重。这种做法可以立即更正食品数额的动物或者是低于或高于自己的目标权重。它也提供了一个方法，以确定是否将大鼠在24小时的间隔保持的权重。此外，还要避免体重在一天中的不同时间大鼠;它会导致体重差的行为表现的巨大差异，不正确的喂养和。 </li><li>在家庭笼所有时间通过​​使用玻璃或塑料瓶的带橡胶塞的顶部和吸管提供淡水。 </li><li>当会话不会进行（ 例如 ，周末，节假日），喂大鼠正常大鼠饲料的配股，以保持体重稳定。称取鼠，以确定是否需要更多的食物，在这些时候保持体重。 </li></ol> 2.设备和软件<ol><li>使用配备有一个鼻子戳键，房子光，和颗粒机（ 即馈线）标准的模块化操作室。</li><li>广场上的中心位置颗粒机两侧的模块化鼻子戳关键;确保该位置仍然在其他操作室和整个研究是一致的。这里，鼻子戳键是在粒料分配器的左侧。 </li><li>放置模块化房子光在腔室的后壁高以便不与其他行为干扰。 </li><li>使用大鼠食物颗粒（ 例如 ，诺伊斯45毫克的颗粒或类似的）进行加固。馈线模型决定了食物颗粒的大小，但大多数老鼠饲养用45毫克的食物颗粒（鼠标馈线通常使用20毫克的食物颗粒）。使用标准饲料颗粒，但其他颗粒可以针对不同的实验操作购买（ 如蔗糖）。 </li><li>通过控制连接到计算机的接口刺激介绍，鼻子戳响应输入，加固交付和数据收集。联系，了解有关书面行为计划的具体信息作者一种编程语言，专门为行为测试（见材料和试剂表 ）设计的。 注:重要的是要记录一个试验按试验的基础上自变量和因变量，包括动物的反应类型（ 例如 ，正确的反应，过早的反应，未命中），响应延迟，并颁发试验次数。当使用不同的菌株，性别或种类，这些变量可以容易地改变在几乎任何类型的啮齿动物的最大化训练。 </li></ol> 3.训练前<ol><li>每个动物分配给操作性室在那里将每一天进行测试，并在约一天的同一时间，在整个实验过程。 </li><li>称量大鼠，并将其放置在与粒料分配器食物颗粒其具体操作性室。将10 - 20食物颗粒在容器中的老鼠吃。 </li><li>对于训练前，设置以下参数低，固定值:foreperiod值= 2秒，超时值= 2秒，保持有限= 10秒。 </li><li>启动所述计算机程序，以控制住宅光的介绍，nosepoke按键照明，并自动食物丸递送。 注意:最好是有行为计划设置为一个级别，允许频繁增援，为广大的由动物产生反应，使得鼻子戳键点亮的时间相对较长的时间（ 如 10秒） 。这增加了，同时它被照亮，从而由计算机程序递送食品增强剂的大鼠会戳密钥的可能性。 </li><li>一旦老鼠完成的食物颗粒，通过加强老鼠当它接近食物容器或鼻子戳键启动塑造过程。这里，手动塑造各大鼠，这意味着，实验者手表老鼠的行为（通过使声音衰减机柜门打开时），并从进料器提供粒料尽快后大鼠发射所需的行为。从上料本身的一个按钮递送小球，通过被连接到馈线，或通过使用通过点击界面上的相应的输出，以控制行为意外事件的计算机软件的手持式开关。 </li><li>使用由连续逼近成形的方法中，对于那些连续逼近到最终期望的行为23行为的变化提供了食物增强剂。例如，一旦将大鼠可靠地从食品容器取回食物颗粒，开始朝向或鼻子周围戳键补强只移动，并且不再加强方法的食品容器。这里，形状大鼠移至左侧检索食物丸后，由于鼻子戳键位于在操作室的食品容器的左侧。 注:即使实验者pretr年初交付期间的强化食品癌宁，所述计算机程序仍在运行和控制房子光，nosepoke键，和馈线。如果一只老鼠刺探键，电脑程序会自动加强它。实验者增强的行为仅仅是那些导致大鼠捅照明键，如嗅键或周围，移动头部附近的键或向它， 等等 。 </li><li>通过计算手动交付颗粒的数量加上由计算机软件自动提供任何颗粒确定训练前会议期间获得的食物量（ 即颗粒传递，因为鼠戳照明键）。通过0.045克乘以这个数来确定总获得食物（ 例如 ，30粒点¯x0.045克=1.35克）。 </li><li>取出老鼠，并把它放回笼子。减法，手，食物的量，从老鼠的日常食物配股行为会赚（见3.7）。喂食物分配的剩余部分大鼠后行为训练。饲料至少30分钟的行为会议结束后立即避免非生产性会议之后笼子食品加固。 </li><li>用温和的消毒剂（ 如肥皂水）日常清洁操作性室。使用70％乙醇节制，因为它可能损坏操作室。每天检查鼻子戳键以便在需要时保持正常的工作状态，并用消毒剂消毒，因为那支或者被锁定到位如果不能迅速修复会破坏动物的性能的关键。 </li><li>形状以这种方式各大鼠直到大鼠发射40 - 对照键50正确计算机增强（未手动或实验者增强）响应，检索各食物丸，并返回到和戳无需额外整形鼻子戳键。通常，大鼠需要约2 - 3 30分钟的会话，达到这些标准（见图2）。 注意:大鼠被可靠地戳密钥和接收的计算机程序运送食物加固后不需要食物颗粒手册交付。 </li></ol> 4. rPVT培训<ol><li>一旦成型，在一个固定的，相对较短的时间间隔foreperiod启动老鼠，直到大鼠完成8出10正确的试验，以在下一次30分钟的会话值（例如，2秒）。对于每个训练期间这些试验，照亮了一段足够长的时间对大鼠做出响应鼻子戳键。在这里，保持亮起9秒的关键;此响应窗口也被称为有限保持（LH）。 </li><li>使用一个相对短的时间进行（TO; 例如 ，2秒），作为过早响应的惩罚，或前鼻戳键的照明响应。该期间，老鼠没有机会获得食物。在这里，TO和foreperiod价值都在以同样的速度，这些培训和变革同样的时间。 </li><l我&gt;一旦老鼠完成8出10正确的试验中，增加foreperiod以及由0.1秒（ 即 2.1），而由0.1秒缩短LH（ 即 8.9）。最好是使用计算机程序，可以在会话过程中自动进行这些调整。 注:当在rPVT训练老鼠，更长的ITI采用，因为老鼠需要更多的时间来消耗食物颗粒。在这里，用30秒的ITI训练和测试雄性C57BL / 6J。此外，由于鼠标可能重达约20克，将在会议期间消耗更少的显著颗粒。因此，有它结束的时候，最大的50颗粒赚缩短一个典型的会议;为提高foreperiod和TO的标准也改为4，满分5正确的审判由0.1秒增加foreperiod和之前。 </li><li>一旦30分钟会话结束，记录最终foreperiod的训练（ 例如 ，4.2 S）期间达成的老鼠，无论是作为preadsheet或通过在一个数据片的手，和食品的获得的量，这是由正确的试验次数乘以0.045克（ 例如 ，100正确试验点¯x0.045克= 4.5克）。减去从大鼠的日常配股这种食物量和饲料老鼠在笼子剩下的食物。 请注意:每日数据文件记录每个鼠杀入决赛foreperiod。以确定在会话获得的食物量的计算可自动通过计算机程序或通过手使用上述公式来完成。获得通过的手从每只大鼠扣除食品是每天的食物分配。 </li><li>第二天，有foreperiod比大鼠在前一交易日结束对价值较低的300毫秒开始30分钟训练课。例如，如果老鼠在4.2讲完，本次会议开始于3.9秒。继续担任老鼠在每个foreperiod完成8出10正确的审判由0.1秒增加foreperiod，UNTIL大鼠达到10个foreperiod。以这种方式，大鼠将经历所有可能foreperiods的。 注:如果只达到9.7 foreperiod - 10秒，TO保持在8秒和LH是1.5秒。 </li><li>在每届会议结束时，通过检查纠正审判，过早的试验和未命中的百分比在此期间检查老鼠的日常表现。在这些培训课程90％的正确反应，平均正确率在整个训练持续增加 - 大鼠常用的40的平均维护。这里，对于各大鼠导出日常数据，为了跟踪日常会话性能计算这些百分比的电子表格。 </li><li>一旦大鼠达到10个foreperiod，开始下一个后续的30分钟会话使用随机时间间隔提出的foreperiods，从7之间foreperiods - 10秒。确保TO和LH分别保持在8和1.5秒。这里，计算机程序控制behaviora升意外事件自动呈现上升foreperiods变量foreperiods一次每只大鼠达到在10秒foreperiod 8出10标准进行切换。 </li><li>一旦大鼠保持与7之间随机赠送foreperiods正确地至少70％ - 10秒，改变程序以随机​​间隔呈现foreperiods，开始用5之间foreperiods - 10秒。在此期间，检查各组大鼠每天的性能，因为这种变化通常在会话中发生的。 </li><li>大鼠保持70％的正确之后，再次更改计划提出的全方位foreperiods的 - 在30分钟会议期间在随机时间间隔（3 10秒）。以类似于4.8的方式，在此期间检查各大鼠的每日的性能，因为这种变化通常是一个会话中发生的情况。 注:计算机软件自动控制行为偶然性使得基于每只大鼠的表现这些变化（ 如 4.7 - 4.9）。只有做出改变的4.9是很重要的，当老鼠的成功率高（&gt; 70％正确的），因为老鼠经常采取及时响应随机，而不是等待正确和出席的刺激战略。如果老鼠的老鼠成功率低，重复4.7 - 4.9。 </li><li>对于收购标准，定义基线表现一样稳定的时候，就在一个星期内4出5日常会话，大鼠的正确反应比例为75％或以上的会议期间过早反应的百分比小于30％。 </li></ol> 5.基本应对措施的数据分析<ol><li>为了采集过程中评估行为表现，采用重复测量方差分析，无论是与会议，时间对任务价值，或Foreperiod价值下文所述的各项性能度量重复的因素。 注:在这里，研究使用这两种内和批间科目的设计，我们所有的动物比较他们的Ø实验条件后WN基准和比较实验动物来控制遵循同样的手法动物。当稳定的行为的基线都实现了，使用的试内设计将限制所需要的大鼠的数目。 </li><li>定义为关键光发病前或钥匙灯正对的第一个150毫秒内响应过早反应。 </li><li>定义正确的反应作为主光源后作出回应亮起，以最小的延迟大于150毫秒，响应时间窗口长度的最大延迟;在这里，1500毫秒。 </li><li>定义缺失（或不作为）作为其主体未作出回应试验。 </li><li>定义从发病光反应潜伏期为经过的时间（毫秒）的鼻子戳关键抑郁症;也被称为反应时间（RT）。 </li><li>总定义为试验过早反应加上正确的反应加命中的数量。 注:在人类PVT表演，过早的资源积水和失误的关注可以说是相当罕见的，研究者最常只报告这些措施的实际原始频率。然而，这是不与大鼠表演的情况。为了允许不同的版本在文献中rPVT的之间的比较，大鼠表现措施这里表示为总试验的百分比。计算机程序的使用，可以记录并标记每个项目的试验按试行随后的分析是首选，因为老鼠会完成每30分钟会超过200次试验，并且常会符合标准移动到另一个步骤在会话中的训练过程。 </li></ol> 6.额外计算的性能措施<ol><li>对于一个30分钟的行为会议上，定义为失误的正确反应，其中RT比平均反应时间为每个老鼠的会议（会议后进行）的两倍;还包括在计算失误无缘试验。 没有TE:在人类PVT，失误通常定义为反应时间（RT）&gt; 500毫秒4。啮齿动物，最好是进行事后比较，以每只鼠的平均RT - 从上述试用通过审上述分析获取的 - 而不是将它们与阈值，由于在从单个大鼠的RT的可变性。这种方法类似于rPVT 15,16的其他版本的出版失误。在这里和其他公布的版本中，失误还包括错过试验（失误=带RTS回应&gt;两次的平均值为RT会话，加上未命中），因为在人类的PVT错过试验被记录为失误，以及。 </li><li> 10秒间隔时，刺激会出现 - 通过计算实际3期间发生过早反应的百分比确定估计误报率。 注:这一措施的使用只在当可能发生刺激的时刻，发生过早的反应的一个子集，即3中发生的过早反应- 10秒foreperiod窗口。这一措施并不包括因为刺激光绝不会在这段时间被照亮前3秒过早反应。在这里，过早的反应这个子集自动从电子表格中的计算机程序都过早的反应来分类的。 </li></ol>

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The authors have nothing to disclose.

本文中所描述的方法导致rPVT演出，在许多方面比较有利，以在人类文献报道的典型PVT性能影响。使用本技术，可以迅速地训练的大鼠进行rPVT，用这些技术获得的性能都受到明确歧视（ 即 ，高水平的正确响应，既过早反应和失效水平低），其特征。另外，在rPVT得到的反应时间分布是十分类似于在人类见到执行PVT 12（ 见图5），因为是的频率既过早响应和失效（ 例如...

人类精神警觉测试（PVT）是一种广泛使用的，用于测量警觉和持续地注意在人类以及验证工具，最初是由丁格斯等人开发的。 1-3评估的反应时间和注意力的稳定性（ 例如 ，在过早反应和注意失误方面的错误）都内的会话作为一个整体和单个进程内的时间。多年来，人类PVT已被修改并更新4-11跟踪在各方面关注的时间变化，并已被证明是在睡眠剥夺疲劳的变化敏感，并且通过使用药物和主体12年龄的影响13。该PVT是一个看似简单的过程，其中一个主题简要介绍了一个画面时的刺激（一般是一个LED号码显示）后2随机及时出现，一般 - 10秒。在人的版本，在编号显示在毫秒递增，并停止在t他触摸屏幕，因此显示拍摄对象的反应时间（RT）。减小在警觉由1表示）减慢反应时间，2）增加的失误（在人类文献称为"遗漏错误"，并且通常定义为在长度&gt; 500毫秒）RT和3）增加过早的响应（称为"委托的错误"或"出师不利"，在人类文学）。也可以与所述PVT用于检查变量如性别和年龄的差异获得的其它措施;对这些措施进行审查，看Basner和丁格斯4。最后，PVT已经在人类风险评估的一般地区采用，并已成功地在大范围，包括军事，航空，铁路等行业，第一反应，以及诸如美国宇航局的极端环境任务极端环境业务领域的使用操作（NEEMO），国际火星500计划项目14，并在国际SPACE站（ISS）。在国际空间站上的PVT被称为"反应自检"，并用来提供个性化的疲劳相关的反馈宇航员（ 例如 ，在RT或注意力失误的变化）。 人类PVT已使用了几十年，作为具有简单的反应时间任务啮齿类版本（这是有些相似）。它已被最近才然而，一个直接啮齿类对应于人PVT已经在文献中报道。佳士得和他的同事描述的版本人类PVT的老鼠，并报告睡眠剥夺15,16的警惕性下降。最近附加的研究已经报道了rPVT 17-19的版本。这些报告描述了以下的各种睡眠剥夺技术持续关注的变化;然而，这些研究中的数据也报道高水平的过早响应（ 例如 ，在一些情况下，respo的总数的40％以上国家科学教育标准）;这样的表现是相当不同的PVT表演与人类。在啮齿动物与人类表演可能是由于在人类与所述PVT的啮齿动物版本使用了特定的参数的差异如此之大的差异;例如，Christie 等。研究采用随机变化的3 - 7秒foreperiod，而一个人的PVT通常采用2 - 10秒foreperiod（虽然见Basner 等 5 3分钟版本人类PVT的一个使用1 - 4秒foreperiod） 。使用相对短foreperiod值常常会导致动物"定时"他们的反应，因此可以促进经由偶然加固，增加的过早响应号码，如已在当前啮齿类rPVT研究的报道。 这里所描述的rPVT的版本是基于我们的先前公布的第20条，并提供所涉及的技术和方法的详细描述。它不同于从在以下几个方面的rPVT的先前公布的版本:1）大鼠用的3可变foreperiod值训练有素 - 10秒，和2）大鼠具有快速响应，因为只有很短的响应窗内的反应（也称为"有限在rPVT以前出版的版本）3.0秒;举办"）刺激开始后进行了加固（1.5秒在本研究中。利用这些修改，以及对不正确简要超时作出答复导致刺激控制的更高水平，通过在准确度显著改进所指示，降低过早响应水平。本报告还描述了在性能变量预测的变化（ 如注意力，即时战略失误）是平行与人类看出，当检查警惕减21，并研究其他性能的措施，包括人类的"时间任务"的效果和响应-当刺激间隔（RSI）是在人类PVT效果观察<s达&gt; 22。 这里所描述的rPVT的最终版本通过打开房子光开始（参见图1）。 3的可变间隔（foreperiod）后 - 10秒过后，鼻子戳键点亮时间最长为1.5秒。 （为了保证foreperiods持续时间的相等分布，而不脱离的，从3至10秒的范围内36个可能值的列表，替换是随机生成的值，是根据200毫秒增量。）的nosepoke键的照明是用于动物的信号响应，并发生之间以下的光发病150至1500毫秒与45毫克沉淀增强的响应。钢筋响应后，无论是鼻子戳按键灯和房子灯被关闭，一个1秒审间的间隔（ITI;房子灯熄灭）随之而来。鼻捅在光前起病产生8秒从被灭火的房子光信号实验的意外超时（TO）。如果1.5秒内资源没有任何反应发生ponse窗口，同时nosepoke光和房子光被断开，并且1秒的ITI接着而来。在随后的审讯中下一个计划foreperiod值开始无论是1秒ITI或8秒TO，现有审判期间发生者为准之后。会话（5天/周）每天进行的，通常由约200次试验，30分钟后结束。这将导致与具有大约7.5秒的时间，平均每次试验。 整形的rPVT稳定的基线性能由1来实现）最初适应大鼠取食物小丸出食物托盘的腔室，2）手工成形大鼠通过加强连续逼近到最终上的鼻子戳键响应鼻子戳反应，以及3）进行日常会话，其中rPVT过程的参数（ 即 foreperiod，ITI，TO，和重点照明倍）正逐渐在一个会议上调整，取决于每个老鼠是如何以及每次会议期间执行（在下面详细描述）。

<table><tbody><tr><td>Modular rat operant chamber with stainless steel grid floor</td><td>Med Associates</td><td>ENV-008</td><td>Med Associates Inc.&lt;br/&gt; PO Box 319&lt;br/&gt; St. Albans, Vermont 05478,&lt;br/&gt; USA&lt;br/&gt; Phone: (802) 527-2343</td></tr><tr><td>Sound attenuating chamber&amp;nbsp;</td><td>Med Associates</td><td>ENV-022MD</td><td>Houses rat operant chamber</td></tr><tr><td>Houselight for rat</td><td>Med Associates</td><td>ENV-215M</td><td></td></tr><tr><td>1&quot; diameter rat nose poke response key</td><td>Med Associates</td><td>ENV-119M-1</td><td></td></tr><tr><td>Pellet receptacle, trough type</td><td>Med Associates</td><td>ENV-200R2M</td><td></td></tr><tr><td>Modular pellet dispenser for rat, 45-mg</td><td>Med Associates</td><td>ENV-203M-45</td><td></td></tr><tr><td>PCI Operating package for up to 8 operant chambers</td><td>Med Associates</td><td>MED-SYST-8</td><td></td></tr><tr><td>SmartCtrl</td><td>Med Associates</td><td>DIG-716P1</td><td>This catalog number has 8 outputs and 4 inputs which is the minimum needed to run the rPVT; SmartCtrl can also be purchased with 16 outputs and 8 inputs for more flexibility</td></tr><tr><td>Med-PC IV software</td><td>Med Associates</td><td>SOF-735</td><td></td></tr><tr><td>PC computer with PCI card slot</td><td>Any manfacturer (&lt;em&gt;e.g.&lt;/em&gt;, Dell)</td><td></td><td>Use of the PCI operating package requires a computer with a PCI card slot. Systems that use PCIe are available. Contact Med Associates for details.</td></tr><tr><td>Dustless Precision pellets 45-mg rodent grain-based diet</td><td>Bio Serv</td><td>FO165</td><td>Bio-Serv&lt;br/&gt; One 8th Street, Suite 1&lt;br/&gt; Frenchtown, NJ 08825, USA&lt;br/&gt; Phone: (800)-996-9908;&amp;nbsp;&amp;nbsp; Standard chow pellets are commonly used. Different pellets (&lt;em&gt;e.g.&lt;/em&gt;, sucrose) can be acquired from Bio Serv.</td></tr></tbody></table>

the rodent psychomotor vigilance test (rpvt): a method for assessing neurobehavioral performance in rats and mice

人类的精神运动警觉测试（PVT）是测量疲劳和持续关注的变化一种广泛使用的程序。本文章介绍了PVT-称为"rPVT"，也就是说措施的关注类似方面啮齿类动物的版本（ 即性能准确性，电机转速，过早的响应，并注意失误）。数据表示当与实验室大鼠使用该证明rPVT的短期和长期的有用性。大鼠轻松学习rPVT，学习执行基本过程需要不到两个星期的培训。收购后，大鼠的表演在rPVT表现出高度的相似性在人类PVT这些相同性能的措施，包括在整个会议时间（即 ，人类的"时间导通的相似之处，注意失误，反应时间，警惕性递减任务"效应），以及对人类所描述的响应刺激间隔（RSI）的效果。因此，第rPVT可用于评估多种上持续关注变量非常类似于人类的PVT性能的效果的极有价值的工具，因此，可用于开发神经行为机能障碍的新的治疗是有用的。

基线鼠rPVT表演随着这里详细的参数，122雄性大鼠的86.7％，达到了平均9.0在最后一rPVT参数（SD = 10.13; N = 122）电脑自动化培训课程（4.1节 - 4.9 rPVT培训;根据我们的经验，只大鼠的约2％将不会获得rPVT）。用目前的方法，大鼠平均73.4％的正确回应，18.6％的失误的注意力，和10.6％过早下rPVT标准的性能参数，他们的第一个完整的会话?...

Watch this Scientific Journal Video about The Rodent Psychomotor Vigilance Test rPVT: A Method for Assessing Neurobehavioral Performance in Rats and Mice at JoVE.com

The Rodent Psychomotor Vigilance Test rPVT: A Method for Assessing Neurobehavioral Performance in Rats and Mice

人类的精神运动警觉测试（PVT）的大鼠的版本描述测量类似于那些与人类PVT，包括如性能准确性，电机转速，过早应对失误在关注人类警惕的各个方面，并测量关注的方面。

鼠类精神运动警觉测试（rPVT）:一个在大鼠和小鼠的神经行为评估性能的方法

The human Psychomotor Vigilance Test (PVT) is a widely used procedure for measuring changes in fatigue and sustained attention. The present article ...

the-rodent-psychomotor-vigilance-test-rpvt-method-for-assessing

Universidad San Sebastian

Research

JoVE Journal

Behavior

The Rodent Psychomotor Vigilance Test (rPVT): A Method for Assessing Neurobehavioral Performance in Rats and Mice

12.0K 次观看.  The Johns Hopkins University School of Medicine. 人类的精神运动警觉测试（PVT）的大鼠的版本描述测量类似于那些与人类PVT，包括如性能准确性，电机转速，过早应对失误在关注人类警惕的各个方面，并测量关注的方面。 

视频: The Rodent Psychomotor Vigilance Test rPVT: A Method for Assessing Neurobehavioral Performance in Rats and Mice

Assessment of Sensorimotor Function in Mouse Models of Parkinson's Disease

Sensitive and reliable behavioral outcome measures are essential to the evaluation of potential therapeutic treatments in preclinical trials for many neurodegenerative diseases. In Parkinson's disease, sensorimotor tests sensitive to varying degrees of nigrostriatal dysfunction are fundamental for testing the efficacy of potential therapeutics. Reliable and quite elegant sensorimotor measures exist for rats, however many of these tests measure sensorimotor asymmetry within the rat and are not entirely suitable for the newer genetic mouse models of PD. We have put together a battery of sensorimotor tests inspired by the sensitive tests in rats and adapted for mice. The test battery highlighted in this study is chosen for a) its sensitivity in a wide variety of mouse models of PD, b) its ease in implementing into a study, and c) its low expense. These tests have proven useful in characterizing novel genetic mouse models of PD as well as in testing potential disease-modifying therapies.

In Parkinson's disease and movement disorders in general, sensitive and reliable behavioral assays are essential for testing novel potential therapeutics. Here, we describe a manageable battery of sensorimotor tests for mice that are sensitive to varying degrees of injury to the nigrostriatal system and useful for preclinical studies.

帕金森氏症小鼠模型的感觉功能评估

Sensitive and  reliable behavioral outcome measures are essential to the evaluation of  potential therapeutic treatments in preclinical trials for many  ...

科研

行为学

Automated, Quantitative Cognitive/Behavioral Screening of Mice: For Genetics, Pharmacology, Animal Cognition and Undergraduate Instruction

We describe a high-throughput, high-volume, fully automated, live-in 24/7 behavioral testing system for assessing the effects of genetic and pharmacological manipulations on basic mechanisms of cognition and learning in mice. A standard polypropylene mouse housing tub is connected through an acrylic tube to a standard commercial mouse test box. The test box has 3 hoppers, 2 of which are connected to pellet feeders. All are internally illuminable with an LED and monitored for head entries by infrared (IR) beams. Mice live in the environment, which eliminates handling during screening. They obtain their food during two or more daily feeding periods by performing in operant (instrumental) and Pavlovian (classical) protocols, for which we have written protocol-control software and quasi-real-time data analysis and graphing software. The data analysis and graphing routines are written in a MATLAB-based language created to simplify greatly the analysis of large time-stamped behavioral and physiological event records and to preserve a full data trail from raw data through all intermediate analyses to the published graphs and statistics within a single data structure. The data-analysis code harvests the data several times a day and subjects it to statistical and graphical analyses, which are automatically stored in the &#34;cloud&#34; and on in-lab computers. Thus, the progress of individual mice is visualized and quantified daily. The data-analysis code talks to the protocol-control code, permitting the automated advance from protocol to protocol of individual subjects. The behavioral protocols implemented are matching, autoshaping, timed hopper-switching, risk assessment in timed hopper-switching, impulsivity measurement, and the circadian anticipation of food availability. Open-source protocol-control and data-analysis code makes the addition of new protocols simple. Eight test environments fit in a 48 in x 24 in x 78 in&#160;cabinet; two such cabinets (16 environments) may be controlled by one computer.

Fully automated system for measuring physiologically meaningful properties of the mechanisms mediating spatial localization, temporal localization, duration, rate&#160;and probability estimation, risk assessment, impulsivity, and the accuracy and precision of memory, in order to assess the effects of genetic and pharmacological manipulations on foundational mechanisms of cognition in mice.

小鼠自动化，定量认知/行为筛选：对于遗传学，药理学，动物认知与本科教学

We describe a high-throughput, high-volume, fully automated, live-in 24/7 behavioral testing system for assessing the effects of genetic and ...

A Fully Automated Rodent Conditioning Protocol for Sensorimotor Integration and Cognitive Control Experiments

Rodents have been traditionally used as a standard animal model in laboratory experiments involving a myriad of sensory, cognitive, and motor tasks. Higher cognitive functions that require precise control over sensorimotor responses such as decision-making and attentional modulation, however, are typically assessed in nonhuman primates. Despite the richness of primate behavior that allows multiple variants of these functions to be studied, the rodent model remains an attractive, cost-effective alternative to primate models. Furthermore, the ability to fully automate operant conditioning in rodents adds unique advantages over the labor intensive training of nonhuman primates while studying a broad range of these complex functions.
Here, we introduce a protocol for operantly conditioning rats on performing working memory tasks. During critical epochs of the task, the protocol ensures that the animal's overt movement is minimized by requiring the animal to 'fixate' until a Go cue is delivered, akin to nonhuman primate experimental design. A simple two alternative forced choice task is implemented to demonstrate the performance. We discuss the application of this paradigm to other tasks.

A fully automated protocol for rodent operant conditioning is proposed. The protocol relies on precise temporal control of behavioral events to investigate the extent to which this control influences neural activity underlying sensorimotor integration and cognitive control experiments.

为感觉运动整合与认知控制实验完全自动化的啮齿动物调理协议

Rodents have been traditionally used as a standard animal model in laboratory experiments involving a myriad of sensory, cognitive, and motor tasks. ...

Behavioral Assessment of the Aging Mouse Vestibular System

Age related decline in balance performance is associated with deteriorating muscle strength, motor coordination and vestibular function. While a number of studies show changes in balance phenotype with age in rodents, very few isolate the vestibular contribution to balance under either normal conditions or during senescence. We use two standard behavioral tests to characterize the balance performance of mice at defined age points over the lifespan: the rotarod test and the inclined balance beam test. Importantly though, a custom built rotator is also used to stimulate the vestibular system of mice (without inducing overt signs of motion sickness). These two tests have been used to show that changes in vestibular mediated-balance performance are present over the murine lifespan. Preliminary results show that both the rotarod test and the modified balance beam test can be used to identify changes in balance performance during aging as an alternative to more difficult and invasive techniques such as vestibulo-ocular (VOR) measurements.

Motor control and balance performance are known to deteriorate with age. This paper presents a number of standard noninvasive behavioral tests with the addition of a simple rotary stimulus to challenge the vestibular system and show changes in balance performance in a murine model of aging.

衰老小鼠前庭系统的行为评估

Age related decline in balance performance is associated with deteriorating muscle strength, motor coordination and vestibular function. While a number of ...

The Attentional Set Shifting Task: A Measure of Cognitive Flexibility in Mice

Cognitive impairment, particularly involving dysfunction of circuitry within the prefrontal cortex (PFC), represents a core feature of many neuropsychiatric and neurodevelopmental disorders, including depression, post-traumatic stress disorder, schizophrenia and autism spectrum disorder. Deficits in cognitive function also represent the most difficult symptom domain&#160;to successfully treat, as serotonin reuptake inhibitors and tricyclic antidepressants have only modest effects. Functional neuroimaging studies and postmortem analysis of human brain tissue implicate the PFC as being a primary region of dysregulation in patients with these disorders. However, preclinical behavioral assays used to assess these deficits in mouse models which can be readily manipulated genetically and could provide the basis for studies of new treatment avenues have been underutilized. Here we describe the adaptation of a behavioral assay, the attentional set shifting task (AST), to be performed in mice to assess prefrontal cortex mediated cognitive deficits. The neural circuits underlying behavior during the AST are highly conserved across humans, nonhuman primates and rodents, providing excellent face, construct and predictive validity.

The goal of this protocol is to perform a behavioral assay such as the attentional set shifting task (AST) to assess prefrontal cortex-mediated cognitive flexibility in mice.

在注意定势转移任务:测量小鼠认知的灵活性

Cognitive impairment, particularly involving dysfunction of circuitry within the prefrontal cortex (PFC), represents a core feature of many ...

Operant Procedures for Assessing Behavioral Flexibility in Rats

Executive functions consist of multiple high-level cognitive processes that drive rule generation and behavioral selection. An emergent property of these processes is the ability to adjust behavior in response to changes in one&#8217;s environment (i.e., behavioral flexibility). These processes are essential to normal human behavior, and may be disrupted in diverse neuropsychiatric conditions, including schizophrenia, alcoholism, depression, stroke, and Alzheimer&#8217;s disease. Understanding of the neurobiology of executive functions has been greatly advanced by the availability of animal tasks for assessing discrete components of behavioral flexibility, particularly strategy shifting and reversal learning. While several types of tasks have been developed, most are non-automated, labor intensive, and allow testing of only one animal at a time. The recent development of automated, operant-based tasks for assessing behavioral flexibility streamlines testing, standardizes stimulus presentation and data recording, and dramatically improves throughput. Here, we describe automated strategy shifting and reversal tasks, using operant chambers controlled by custom written software programs. Using these tasks, we have shown that the medial prefrontal cortex governs strategy shifting but not reversal learning in the rat, similar to the dissociation observed in humans. Moreover, animals with a neonatal hippocampal lesion, a neurodevelopmental model of schizophrenia, are selectively impaired on the strategy shifting task but not the reversal task. The strategy shifting task also allows the identification of separate types of performance errors, each of which is attributable to distinct neural substrates. The availability of these automated tasks, and the evidence supporting the dissociable contributions of separate prefrontal areas, makes them particularly well-suited assays for the investigation of basic neurobiological processes as well as drug discovery and screening in disease models.

The ability to assess executive functions such as behavioral flexibility in rats is useful for investigating the neurobiology of cognition in both intact animals and disease models. Here we describe automated tasks for assessing strategy shifting and reversal learning, which are particularly sensitive to disruptions in prefrontal cortical networks.

操作性程序，以评估大鼠的行为灵活性

Executive functions consist of multiple high-level cognitive processes that drive rule generation and behavioral selection. An emergent property of these ...

Neurodevelopmental Reflex Testing in Neonatal Rat Pups

Neurodevelopmental reflex testing is commonly used in clinical practice to assess the maturation of the nervous system. Neurodevelopmental reflexes are also referred to as primitive reflexes. They are sensitive and consistent with later outcomes. Abnormal reflexes are described as an absence, persistence, reappearance, or latency of reflexes, which are predictive indices of infants that are at high risk for neurodevelopmental disorders. Animal models of neurodevelopmental disabilities, such as cerebral palsy, often display aberrant developmental reflexes, as would be observed in human infants. The techniques described assess a variety of neurodevelopmental reflexes in neonatal rats. Neurodevelopmental reflex testing offers the investigator a testing method that is not otherwise available in such young animals. The methodology presented here aims to assist investigators in examining developmental milestones in neonatal rats as a method of detecting early-onset brain injury and/or determining the effectiveness of therapeutic interventions. The methodology presented here aims to provide a general guideline for investigators.

Behavioral testing is the gold standard for determining outcomes following brain injury, and can identify the presence of developmental disabilities in infants and children. Neurodevelopmental reflexes are an early indicator of these abnormalities. A host of easily accomplished developmental reflex tests in the neonatal rodent were developed and described here.

神经发育复检新生幼鼠

Neurodevelopmental reflex testing is commonly used in clinical practice to assess the maturation of the nervous system. Neurodevelopmental reflexes are ...

Battery of Behavioral Tests Assessing General Locomotion, Muscular Strength, and Coordination in Mice

Behavioral testing is used in pre-clinical trials to assess the phenotypic effects and outcomes that a particular disease or treatment has on the animal&#39;s wellbeing and health. There are numerous behavioral tests that may be applied. We selected a test for general locomotion, the open field test (OFT); a test for muscular strength, the mesh test (MT); and a test for coordination, the rotarod test (RR). Testing can be accomplished on a weekly or monthly basis. As a test for general locomotion, the OFT works by objectively monitoring movement parameters while the mouse is in an open field apparatus. The field is generally a 2&#39; x 2&#39; box, and the movements are recorded through laser sensing or through video capture. The mouse is placed in the center of the open field and allowed to move freely for the test. The MT uses the latency for a mouse to fall off an inverted screen as a measure of muscular strength. A mouse is placed on a screen, which is inverted over a clear box, and is timed for their latency to fall. Three trials are performed, with the best of the three trials scored for that day. A score of 60 s is the maximum time a mouse is left inverted. Mice are given a 5-min rest period between mesh test trials. Lastly, an accelerated protocol on the RR assesses motor coordination and endurance. During a trial, a mouse walks on a rotating rod as it increases in speed from 4 rpm to 40 rpm over 5 min. The trial ends when the mouse touches the magnetized pressure sensor upon falling. Each mouse undergoes three trials, and the best trial is scored for that day. This combined behavioral data allows for the global assessment of mobility, coordination, strength, and movement of the test animals. At least two out of the three behavioral testing measures must show improvement for an animal to qualify as having overall improved motor function.

Behavioral testing is used in pre-clinical trials to assess the phenotypic effects of a disease or treatment on an animal's wellbeing. In order to globally assess motor functioning, we selected tests for general locomotion, muscular strength, and coordination: the open field test, the mesh test, and the rotarod test, respectively.

用于评估一般运动、肌肉力量和小鼠协调性的行为测试电池

Behavioral testing is used in pre-clinical trials to assess the phenotypic effects and outcomes that a particular disease or treatment has on the ...

Behavioral Assessments of Spontaneous Locomotion in a Murine MPTP-induced Parkinson's Disease Model

Parkinson&#8217;s disease (PD) is a common neurodegenerative disorder disease, causing the phenomenon of shaking, rigidity, slowness of movement and dementia. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) can lead to some Parkinson&#8217;s-like symptoms by destroying dopaminergic neurons in the substantia nigra of the brain. It has been thus used to establish PD models in various animal studies. Here, mice receive MPTP injections (20 mg/kg/day) for seven days and the behavioral tests are performed on the eighth day. This model is adapted efficiently in the study of PD. The behavioral tests here include the cylinder test and the open field test. The cylinder experiment is used to detect the animals&#8217; ability to lift their front paws when put into a different environment. As the PD model mice show arching&#8212;the mouse arches its back&#8212;the number of paw liftings decrease. This test is easy to execute. The open field test is used to detect the amount of time the mice spend on running, walking, and remaining immobile. We analyze animals&#8217; movements in open field using software and obtain data. Lastly, we use L-DOPA, one of the most commonly used PD drugs, as one example to show how to apply this model to the study of PD drugs. Our results indicate that MPTP neurotoxicity induces motor deficit which can be mitigated by L-DOPA.

We describe establishment of a murine model for Parkinson's disease using MPTP, and behavioral assessments using cylinder and open field tests to measure motor function. We then use L-DOPA as one example to show how to apply this model in the study of PD drugs.

小鼠 mptp 诱导的帕金森病模型中自发性运动的行为评估

Parkinson&#8217;s disease (PD) is a common neurodegenerative disorder disease, causing the phenomenon of shaking, rigidity, slowness of movement and ...

A Behavioral Test Battery for the Repeated Assessment of Motor Skills, Mood, and Cognition in Mice

Pharmacological and toxicological studies in neurodegeneration require comprehensive behavioral analysis in mice because motor dysfunctions and dysfunctions in mood and cognition are common and often shared symptoms in neurodegenerative diseases. Shown here is a behavioral test battery for motor, mood, and cognition, which can be repeatedly tested in a longitudinal study. This battery assesses the overall behavioral phenotype in mice by examining each domain of behavior with at least two independent well-accepted tests (i.e., open-field test and rotarod test for motor function, social interaction test, elevated plus maze test, and forced swim test for emotional function, and Morris water maze test and novel object recognition test for cognitive function). Therefore, this sensitive and comprehensive test battery is a powerful tool for the study of behavioral alternation in neurodegeneration.

A comprehensive behavioral test battery of motor skills, mood&#8212;including social interaction, depression, and anxiety&#8212;and cognition is designed for the repeated assessment of neurodegeneration-related behavioral changes in mice.