Name: bioluminescence-based tumor quantification method for monitoring tumor progression and treatment effects in mouse lymphoma models
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我们感谢科德利埃俱乐部研究中心（CEF，巴黎，法国），Genethon（埃夫里，法国） 和Genopole（CERFE，埃夫里，法国）的动物设施。我们感谢乔·安·卡恩对她的手稿仔细阅读。这项研究是由研究所国家德拉桑特的Et德拉Rechercher MEDICALE，巴黎第五大学，皮埃尔与玛丽·居里大学，协会倒拉RECHERCHE癌症中心，突尼斯方向兴业德拉RECHERCHE科学研究，法突尼斯CMCU支持项目，ThématiquesIncitatives德Genopole（ATIGE）的资金操作。 JC是由生命科学学院博士生前沿，并通过从INCA（国立研究所杜癌症）的奖学金支持。 RBA是从DGRS-INSERM和CMCU助学金获得者。 SD从国家研究所杜癌症获得了资助。

所有涉及小鼠过程符合欧盟的指导方针，法语条例动物实验（编号2001-464农业部法令，2001年5月），和国家研究所德拉桑特等德拉RECHERCHE MEDICALE（INSERM）委员会的指导方针动物研究，并分别由有关地方委员会批准（查尔斯·达尔文伦理委员会的动物实验，巴黎，法国;许可证号:P3 / 2009/004）。 1.细胞的制备<ol><li>生长小鼠B淋巴瘤细胞系A20.IIA-luc2在补充有10％胎牛血清，100μg/ ml的青霉素，100微克/ ml链霉素，10mM的丙酮酸钠，50μM2-巯基乙醇，和0.50的RPMI-1640 Glutamax的培养基毫克/ ml潮霉素B. </li><li>维持细胞培养物，在37℃，5％CO 2和改变介质每两至三天。收获5毫升用吸管一天细胞悬浮液更换培养基后。 </li><li>旋转单元3005;克10分钟，悬浮细胞在3ml无菌磷酸盐缓冲盐水（PBS）。重复此步骤两次以洗涤细胞。 </li><li>加载Malassez计数室之前混合15微升30微升台盼蓝标记细胞悬液。与式计算细胞浓度:浓度（细胞/ ml）=细胞在计数网格* 3 * 1000号。 </li><li>旋转细胞一次，在300×g离心10分钟。用移液管除去上清液。用C在步骤1.4计算出的浓度），细胞的数目是N = C * 3。 </li><li>计算无菌PBS 1×中的5×10 7个细胞/ ml与下式的浓度，以获得细胞悬液的必需体积:PBS容积（毫升）= N /（5×10 7）。暂停将细胞沉淀（来自步骤1.5）在无菌PBS 1X的前一句中计算出的体积）。细胞悬浮液A在SCL模型中使用（ 在体内注射体积为100微升:5×10 6细胞S）。 </li><li>吸管10微升细胞悬浮液A和添加90微升无菌PBS在1.5ml管中，在每毫升5×10 6个细胞，得到100微升细胞悬浮液B辑。细胞悬浮液B是在PIOL模型中使用（ 在体内注射体积是2微升:1×10 4个细胞）。 </li></ol> 2.荧光素<ol><li>在一个50毫升管稀1克D-萤光素钾盐粉末在30毫升无菌PBS 1X和摇动几秒钟，以溶解聚集体。 注:由于荧光素是光敏感，准备在黑暗的1.5毫升离心管500微升等分。 </li><li>储存在-20℃的等分试样。 注:等分试样可存放数个月。 融化后，将等分试样不能在+ 4℃下储存超过1天。冻融循环是优选在4℃下贮存。 </li><li>注入100微升D-萤光素钾盐溶液腹膜内为每个成像阿萨年。 注意:此溶液相当于每只小鼠3.3毫克的剂量为150毫克/公斤。 </li></ol> 3.麻醉剂混和麻醉<ol><li>制备通过在无菌PBS中1×混合氯胺酮120 mg / kg和赛拉嗪6毫克/公斤的麻醉剂溶液。 </li><li>注入60微升麻醉剂混腹腔与将25g针头每个成像试验。手术（与​​PIOL或SCL模型），注入80微升混合物，得到更深入的麻醉。将鼠标放回笼子。 </li><li>当鼠标不动的出现，从笼中取出，并轻轻挤压手指之间的鼠标的腿。如果鼠标与逃避反射反应，等待几分钟。重复上述动作，直到鼠标没有反应，这证实了满意的麻醉。 </li><li>把鼠标放在一个保温盘或下一个变暖的光。 </li><li>应用眼膏，以避免麻醉的成像试验或SCL在手术过程中眼睛干涩。应用手术的PIOL模型后眼膏。 </li></ol> 4.手术和细胞接种注:在保温盘或下一个变暖的光执行所有外科手术，在1型微生物安全柜在动物生物安全2级设施。在本节中使用的所有外科手术刀具使用前高压灭菌。 <ol><li> 皮下淋巴瘤型号: <ol><li>制备100微升在1-ml注射器与地下25针在步骤1.6中得到的细胞悬浮液。轻轻挤压上的手指之间的侧面的小鼠皮肤，在注射部位。插入针恰好进入皮肤褶皱。为确保皮下注射，不针放在深入到组织。 <ol><li>注入细胞进入皮褶。观察有无在皮肤下会出现一个小液体球，以确认注射正确执行。 </li></ol></li></ol></li><li> PIOL模型: 注:此PROCE杜热要求2的运营商，这里称为操作者1和操作2。 <ol><li>结膜去除: <ol><li>具有操作员1处解剖显微镜下鼠标。轻轻按压与眼睛的两侧的手指来清除它。保持这个姿势。 </li><li>必须通过解剖显微镜操作2升书籍。握一把小单手钳的结膜;用另一只手，切只是钳子下面结膜用小一对外科剪刀。 </li><li>具有操作员1释放步骤4.2.1.1按下鼠标的眼睛） </li></ol></li><li>细胞注射: <ol><li>准备一个10微升钝精密注射。通过它抽灭菌去离子水清洗。重复两次或三次，以确保有在注射器无气泡。然后准备2微升细胞悬浮液的注射。 </li><li>具有操作员2轻轻按压与手指上Ë手眼的每一边将其清除。保持这个姿势。 </li><li>有操作员1夹持眼用小钳子在一方面边缘，将其轻轻地向后伸展的组织。 </li><li>必须通过解剖显微镜操作2升书籍。用另一只手，使得在同一个32克针鼠标的劣眼球的小孔。 </li><li>具有操作员2放下针，拿起（用同一只手）精密注射器和针头插入步骤4.2.2.4所做的洞。 </li><li>对用另一只手的注射器活塞操作1推。 </li><li>有操作员2 V erify与在注射器中的细胞悬浮液已在眼球内被正确地喷射出的解剖显微镜（液体流很容易观察到的）。 </li><li>具有操作员2 R emove的精密注射。 </li><li>具有操作员1新闻稿的一个边缘进制眼睛笼罩在步骤4.2.2.3） </li><li>具有操作员2新闻稿眼睛的两侧步骤4.2.2.2按下） </li><li>立即应用眼膏。 注意:如果被正确执行的所有步骤，鼠标不应该在此过程中流血的。 </li></ol></li></ol></li></ol> 5.生物发光成像 - 0天注:注入小鼠的所有产品必须在RT注射前。肿瘤细胞后，已经接种，并且在动物仍在麻醉，进行这些步骤的成像。 <ol><li>打开相机，打开采集软件。通过点击"初始化"按钮初始化相机，阶段，和透镜。初始化将需要10到15分钟，以完成。 </li><li>注100微升的D-荧光素钾盐将25g针腹腔解决方案。不要静脉管理它。如果intravenou行政体系所需的任何原因，D-荧光素钠盐 ，必须使用而非D-萤光素钾盐 。 注:萤光素是在该浓度的过量反应物;因此，生物发光信号到达后3〜7分钟的高原，持续超过30分钟。 </li><li> 10分钟 D-荧光素注射后13，将标的鼠标在成像仪。鼠标放置在其自然的位置，其对相机后背 ，在平坦的位置成为可能。这一立场是自然的，容易复制。 </li><li>勾选自动曝光功能，点击采集获取动物的背部 （后）的形象，与自动曝光功能。 注:自动曝光功能，通过从1秒曝光的图片计算它优化曝光时间。如果鼠标的生物发光信号是负的或非常低的，最佳曝光时间可能是自动设定为超过20分钟。在这种情况下，为8至10分钟的曝光时间可以是一个良好的折衷。曝光时间可以被手动设置，但图像不能包含饱和的像素 。 </li><li>转动鼠标以暴露鼠标到相机的正面 。尽量扁平化鼠标和传播其前肢，使它们不会阻塞胸前。 </li><li>采集动物的正视图像。验证自动曝光复选框被选中还是并在"采集"按钮，再次点击。 注:前图像会在背部图像之前被收购，反之亦然。正面和背面的图像的曝光时间可以根据鼠标的每一侧的相对强度是不同的。它正在使用的自动曝光功能时自动计算。量化只使用光子通量（ 每秒光子）和不依赖于曝光时间。 </li><li>把鼠标放在变暖高原e或下一个变暖的光，直到它从麻醉中恢复，然后将其放回笼子里。 </li></ol> 6.生物发光成像 - 0天之后<ol><li>打开相机，初始化相机，阶段，和透镜如在步骤5.1）。 </li><li>麻醉小鼠的氯胺酮（120毫克/千克）/赛拉嗪（6毫克/公斤）混合物的60微升（见步骤3.1至3.5）腹膜内注射。 注:麻醉的这种方法允许成像每5天。执行日常成像，需要麻醉并适用于本麻醉剂使用生物发光成像，使用异氟醚的方法。 </li><li>采集动物的正面和背面的图像，使用自动曝光功能，如在步骤5.5）和5.6）。处理鼠标按步骤5.7）。 </li></ol> 7.生物​​发光定量与图像分析注:luminoscore方法基于图像analysi秒。一旦图像已经根据上述步骤被收购，量化可以在任何时间执行（采集后立即含），以在每个时间点一luminoscore到每只小鼠关联。 <ol><li>通过点击"查看"菜单上显示"工具选项板"，然后点击"工具选项板"如果尚未显示出来。点击工具面板中的"投资回报工具"选项卡。选择"轮廓"按钮，然后选择"免费抽奖"。 </li><li>通过前视图下鼠标的边缘手动标记感兴趣区域（ROI）周围的鼠标的地区。与电脑鼠标右键单击关闭轮廓。 </li><li>点击"查看"菜单，然后选择"ROI测量"上。确保"光辉（光子）"中的"测量类型"中选择了"ROI测量"窗口左下角的滚动菜单。如果不是，选择它。然后测量光子FLUX（PH / s）的记录在"总光通量[P / S]"框中的值。 注意:不要使用辉耀或者其他单位，是相对于投资回报率的表面积。 </li><li>重复步骤7.2）和7.3）的后视图 。 </li><li>总结从正面和背面视图得到的两个光子通量的值。结果是luminoscore。 </li><li>比较的值使用适当的统计检验每个组（非参数双尾Mann-Whitney检验，例如）14。 </li></ol>

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The authors have nothing to disclose.

由器官和组织的光吸收保持生物发光成像的限制，虽然这种限制是固有的任何光学成像模态。在我们的方法的上下文中，预期的解剖结构上的luminoscore的影响具有低可变性提供的研究是在一个给定的模型（位置和鼠标链）进行，允许随后的比较。生物发光不需要激励光，因此在体内成像比荧光更适于全身。 空间分辨率也是生物发光成像的限制，并从其中的生物发光的光子发射的脏器精确位置仍然困难。然而，该模型的良好的知识可以在肿瘤部位的定性位置帮助。在此基础生物发光法的唯一输出是luminoscore。位置不影响luminoscore因为INTERES的手动加价指定地区T（ROI）覆盖了整个鼠标。最后，萤火虫萤光素酶需要氧气。因此，生物发光成像通常会低估坏死肿瘤。再次，需要在模型的这个方面的一个很好的了解。 在本文中，我们的目标不是在评估的CpG的功效作为抗肿瘤的药物（这已被证明7,9,11），但描述允许生物发光数据集进行比较的方法。 我们确实描述量化旨在帮助标准化为在不同的地方在不同的时间比较不同试验中获取协议肿瘤负荷的方法和不需要计算机计算。以确保重现性和正确光子通量定量，所述成像设备必须与自制设备的光基准进行校准或所推荐的提供者的商业设备。 我们的协议需要认真注意的几个关键points:（一）首先，将小鼠麻醉的质量是用于获得清晰的图像，尤其是在具有长曝光时间的情况下的关键。 （二）该量化单元必须始终是光子通量，因为辐射取决于每个小鼠的表面积，并且可能是不相关的，用于比较不同的小鼠。 （三）的生物发光图像必须不含有饱和的像素，因为这些会偏压luminoscore。 （四）返回和前采集需要收集从肿瘤部位（ 即，前采集不一定检测来自肿瘤的背面光子）发射的所有光子。各种不同的ROI附图luminoscore方法的发展过程中进行了测试。仅手动加价产生更有可能是统计学显著（数据未示出），结果令人满意。 Inoue等推荐的75毫克萤光素/ kg的剂量13。通过使用剂量为150毫克/公斤，而不是，成像的定时之后荧光素的管理保持不变，我们要确保高原持续整个长时间曝光的收购。荧光素必须在整个收购过程中过量的反应物。根据不同的模型，感兴趣的区域可以适于，如我们在我们提请每只动物2的ROI在SCL模型显示。在SCL模型中，治疗被注射时，肿瘤在其最大的直径，以限制可变性达到0.5厘米的植入。根据不同的小鼠中，肿瘤可能已经生长不同。标准化和比较小鼠，我们决定使用，揭示两翼肿瘤的相对生长处理和非处理侧之间的比率。 如果没有信号从注入小鼠预期为正，观察或者（i）细胞的数量是非常低，该信号是检测阈值以下;或（ii）小鼠缺乏氧气，需要立即治疗。 几个定量bioluminesc的ENCE分析由不同的作者描述需要复杂的计算和仪器（ 例如 ，3D生物发光断层扫描）接近发出的生物发光的光子5,15的绝对定量。另外，对再现量化生物发光，特别是在肿瘤模型中，与2D生物发光成像的方法没有达成共识。我们的目的是规范图像采集协议，以限制用户的依赖。 CpG的原位注射接种肿瘤后两款车型降低肿瘤负荷。该luminoscore方法可以作为监测肿瘤免疫治疗的其他车型肿瘤负荷的工具。监测肿瘤负荷提供了一个可以提高我们的肿瘤的生长和转移性机制的理解不与肿瘤微环境的干扰一种非侵入性的方法。这和不使用这种方法的治疗有反应的动物的识别是通过S的增强验证uccessful肿瘤细胞注射在实验的开始。 在这里，我们使用A20.IIA-luc2细胞表现出luminoscore方法的适应性在SCL模式。然而，这种方法可以使用其它细胞系，只要它们表达荧光素酶，或适用于任何其他肿瘤模型中的T细胞研究（肿瘤特异性细胞毒性T细胞，调节性T细胞等 ）的情况下。在罕见疾病的基因治疗的上下文中的体内基因转移的监视也可以使用luminoscore方法完成。 最后的数据显示，基于生物发光luminoscore方法能够比较实验之间，提供了灵活性和适应性，具体的实验需要，是无创纵向临床前研究的有用工具。

早期肿瘤细胞的检测仍是一个挑战，是用于增强癌症治疗功效的关键。 体内生物发光成像（BLI）是一个非常敏感的，非侵入性的光学技术，广泛用于小动物监测肿瘤。萤火虫萤光素酶表达细胞通常用于此类实验1,2。此氧氧化D-萤光素与分子氧，但需要两个辅因子-镁离子和三磷酸腺苷3。萤火虫萤光素酶更适合于比海肾萤光素酶4，因为它的量子产率是更高的体内成像。 该氧化的衬底 - 氧化萤光素 - 自发发射光子返回到其基本状态，然后变为无效。所发射的光子具有围绕530nm的最大波长。高灵敏度摄像机可以从一个小动物的内部检测所述发光光子和提供，使得它POSS图像IBLE来定位肿瘤细胞。 通过光子计数准确量化肿瘤负荷的能力可以作为量化处理功效的强有力的和灵敏的工具。因为治疗效果可以越早检测，这种敏感性可能使人们有可能确定在哪个处理成为有效的确切时刻。 总发射光子的绝对定量是非常复杂的。收集光子的数目取决于肿瘤的深度和在光子通过发射的器官。基于组织吸收系数校正系数可以计算出如 ​​图5所示 ，但肿瘤细胞数目的绝对定量需要知道由每个肿瘤细胞发射的光子的数量。而且，萤光素酶表达，像许多报告基因（ 例如 ，荧光蛋白）不是均匀的，即使是在从一个单一的克隆6衍生的细胞群。路西法的数量在细胞中酶蛋白不能被精确地计算。标准化实验条件成立从而出现一个可靠的半定量分析的关键。 我们采用的方法luminoscore两个不同的小鼠淋巴瘤模型7,8,9。在这些模型中，同基因的肿瘤细胞注射到眼睛或皮肤获得，分别一次眼内淋巴瘤（PIOL）模型和皮下淋巴瘤（SCL）模型下。在每个这些原位模型，处理是在就地施用时，肿瘤接种PIOL七天后，当肿瘤已经达到在SCL其最大直径为0.5到0.7厘米我们使用了luminoscore方法监测原位 CpG疗法的效果，先前显示为7,10,11有效。 CpG的是一个寡核苷酸序列和TLR9，一配体，其反过来是由众多的CE表达的细胞内受体免疫系统的LLS，包括树突细胞，B淋巴细胞，单核细胞，和天然杀伤细胞。的CpG-DNA是包含CpG基（CG）的免疫刺激基序的20聚体的DNA序列;控制（ODN控制）是一样的20聚体的DNA序列，所不同的是免疫刺激的CG序列被反转（GC）。在我们所研究的鼠淋巴瘤TLR9接合诱导凋亡10，激活免疫系统12，从而显著降低肿瘤负荷7,11。 在这里，我们描述了量化肿瘤负荷，并通过生物发光图像处理响应的标准方法。此方法依赖于成像过程的不同方面，从采集到的分析，以优化的可靠性，重现性，非用户的依赖，以及统计学意义。生物发光定量指标分配给每个鼠标;这个值，我们称之为luminoscore，可以进行比较，不仅动物，但人之间所以实验之间。 在这项工作中，我们着重于生物发光成像的过程，以及由luminoscore方法图像量化。我们显示用于验证注射，监测肿瘤负荷，并评估原位癌症治疗的功效本方法的有效性。每个点都是在代表结果说明使用不同的小鼠模型突出luminoscore方法的适应性实验。

<table><tbody><tr><td>CpG 1826</td><td>Invivogen</td><td>Sequence: 5_-TCCATGACGTTCCTGACGTT&lt;br/&gt; Catalog number: tlrl-1826</td><td></td></tr><tr><td>ODN 1826 control</td><td>Invivogen</td><td>Sequence: 5_-TCCATGAGCTTCCTGAGCTT&lt;br/&gt; Catalog number: tlrl-1826c</td><td></td></tr><tr><td>D-luciferin potassium salt</td><td>Interchim</td><td>Catalog number: FP-M1224D</td><td></td></tr><tr><td>Ketamin</td><td>Virbac, France</td><td></td><td></td></tr><tr><td>Xylazin</td><td>Bayer Healthcare</td><td>Rompun 2%</td><td></td></tr><tr><td>A20.IIA-luc2 cell line</td><td></td><td>A20.IIA transfected with pGL4.50[luc2/CMV/hygro]&lt;sup&gt;7&lt;/sup&gt;&lt;br/&gt; (Promega E1310)</td><td></td></tr><tr><td>Mice</td><td>Balb/cByj</td><td>Six-weeks old females from Charles River</td><td></td></tr><tr><td>IVIS lumina Biolumienscence imager</td><td>Perkin Elmer</td><td></td><td></td></tr><tr><td>Living Image software</td><td>Perkin Elmer</td><td>Used for measuring photon flux on images and drawing ROIs</td><td></td></tr><tr><td>R software (opensource)</td><td>R-project</td><td>Used for statistic tests</td><td></td></tr><tr><td>Hamilton Precision Serynge 10 &amp;micro;l&amp;nbsp;</td><td>Hamilton</td><td>Product number 7642-01100</td><td></td></tr><tr><td>Eye ointment</td><td>Lacrinorm</td><td></td><td></td></tr><tr><td>Dissecting microscope</td><td>ZEISS</td><td>Stemi 305</td><td></td></tr></tbody></table>

bioluminescence-based tumor quantification method for monitoring tumor progression and treatment effects in mouse lymphoma models

Although bioluminescence imaging (BLI) shows promise for monitoring tumor burden in animal models of cancer, these analyses remain mostly qualitative. Here we describe a method for bioluminescence imaging to obtain a semi-quantitative analysis of tumor burden and treatment response. This method is based on the calculation of a luminoscore, a value that allows comparisons of two animals from the same or different experiments. Current BLI instruments enable the calculation of this luminoscore, which relies mainly on the acquisition conditions (back and front acquisitions) and the drawing of the region of interest (manual markup around the mouse). Using two previously described mouse lymphoma models based on cell engraftment, we show that the luminoscore method can serve as a noninvasive way to verify successful tumor cell inoculation, monitor tumor burden, and evaluate the effects of in situ cancer treatment (CpG-DNA). Finally, we show that this method suits different experimental designs. We suggest that this method be used for early estimates of treatment response in preclinical small-animal studies.

该Luminoscore方法可以用来验证肿瘤细胞注射 在涉及少数肿瘤细胞的注射模型或当注射部位不允许喷射的视觉验证，它可以是非常困难的，以确保注射液的质量。该luminoscore方法作为一种快速，便捷的工具来验证程序的质量和方便即时的一切都正确的确定。在这两个模型中，肿瘤是早在注射（图1A），10分钟后检测。图像单独足以验证肿瘤细胞存在于正确的位置。然而，量化图像提供注射的异质性的想法。的散点图（图1B）清楚地显示（黑点）的动物之间的差是接收和没有重新人为对象（红线）注射。有趣的是，在将SCL模型所获得的信号比在PIOL模型高100倍;这一发现与注射细胞的数目（5×10 6和1×10 4个细胞，分别地）相一致。 <img alt="图1" src="/files/ftp_upload/53609/53609fig1.jpg" /> 图1.验证不同小鼠淋巴瘤模型注射该 luminoscores测量不同型号淋巴瘤肿瘤细胞接种后10分钟。（A）两种型号的代表图像。（B）Luminoscore在不同型号的每个动物。红线对应于肿瘤细胞接种前10只动物测得的平均背景噪声;虚线是平均值+/-标准差。对于每个模型，所有的动物可以从背景噪声区别开来。在PIOL模型中，1×10 4个细胞INOCulated右眼的玻璃体，并在SCL模型，5×10 6细胞皮下注射到小鼠的各个侧面。 <a href="https://www-jove-com.bdigitaluss.remotexs.co/files/ftp_upload/53609/53609fig1large.jpg" target="_blank">请点击此处查看该图的放大版本。</a> 监测肿瘤负荷和治疗反应 该luminoscore也是用于研究肿瘤的生长和治疗功效的强有力的工具。 图2A示出了在控制肿瘤生长的监测的代表性图像和所述CpG处理PIOL基。小鼠每1×10 4个肿瘤细胞接种和治疗原位给药第7天的图像表明，在足够的时间（28天）后，转移开始出现的对照组中使用。尽管原发性肿瘤并未敏感治疗，更少所述CpG处理组（表1）中观察到转移。各组（图2B）中的肿瘤负荷进行定量分析表明CpG的减缓肿瘤的发展，产生经处理的和在28天（非参数双尾Mann-Whitney检验p值= 0.0079对照组之间的统计学显著差异）。尽管如此，肿瘤仍然生长在处理的小鼠中，其中没有存活（未示出数据）。这一发现与先前的报道一致:中央人民政府对初级眼肿瘤10没有显著影响。我们这里的ODN和CpG的组间的显著效果来自于转移生长抑制。 <img alt="图2" src="/files/ftp_upload/53609/53609fig2.jpg" /> 图2.监测肿瘤负荷和治疗反应。 （A）的两个基团的代表图像（ODN控制和CpG处理）○˚FPIOL的小鼠。（b）监测肿瘤负荷的luminoscore。作为对散点图所示，CpG基对luminoscore效果在第28天显著在这两个基团，该方法使得有可能以监测肿瘤负荷并测量轻微但显著（p值= 0.0079）CpG-的效果DNA，这可能没有被单独图像中检测到。 <a href="https://www-jove-com.bdigitaluss.remotexs.co/files/ftp_upload/53609/53609fig2large.jpg" target="_blank">请点击此处查看该图的放大版本。</a> <table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always"><tbody><tr><td> 组 </td><td> 老鼠 </td><td> 转移的存在 </td><td> 位置 </td><td> 光子通量（PH / s）的 </td></tr><tr><td rowspan="5">的CpG </td><td> 1 </td><td>没有</td><td> X </td><td> X </td></tr><tr><td> 2 </td><td>是</td><td&gt;眼睛引流lymp节点</td><td> 9.32E + 05 </td></tr><tr><td> 3 </td><td>没有</td><td> X </td><td> X </td></tr><tr><td> 4 </td><td>没有</td><td> X </td><td> X </td></tr><tr><td>五</td><td>是</td><td>眼引流lymp节点</td><td> 2.21E + 07 </td></tr><tr><td rowspan="5"> ODN </td><td> 1 </td><td>是</td><td>眼引流lymp节点</td><td> 1.06E + 07 </td></tr><tr><td> 2 </td><td>是</td><td>眼引流lymp节点</td><td> 7.25E + 07 </td></tr><tr><td> 3 </td><td>是</td><td>眼引流lymp节点+对侧</td><td> 7.64E + 08 </td></tr><tr><td> 4 </td><td>是</td><td>眼引流lymp节点+对侧</td><td> 1.74E + 09 </td></tr><tr><td>五</td><td>是</td><td>眼引流lymp节点+对侧</td><td> 9.76E + 07 </td></tr></tbody></table> 表1中。T他Luminoscore方法可以适用于不同的途径该luminoscore方法不限于单一类型的小鼠模型; 图3示出，它可以适应不同的小鼠模型。在SCL模型，二肿瘤接枝在小鼠的每一侧。处理是在原位施用到单个侧在第0天，和对侧肿瘤作为它的控制（图3A）。因此，二ROI被每只小鼠得出:一个用于每个侧面（图3C）。在处理的和对照侧的luminoscore之间的比率描述了每个肿瘤的相对过程。该比率被设定为1在第0天，在施用治疗时。我们观察到治疗给药13天后在这个比率显著减少（ 图3D;非参数双尾曼-惠特尼检验p = 0.004）。这种减少表明，处理过的侧肿瘤已被再吸收，在代表性生物发光图像（图3B）所示。 <img alt="图3" src="/files/ftp_upload/53609/53609fig3.jpg" /> 图3.适应的Luminoscore方法与两个主要的肿瘤部位模型。 （A）的SCL测定的实验设计。所述小鼠用在每个侧面的两个主要的肿瘤注射。一侧注入任一的CpG-DNA或ODN控制，而另一侧用PBS，以作为它的控制。（B）中 ，在0天和13天的治疗的CpG处理的和对照小鼠的代表性图像给药关于第0天的肿瘤生长受到抑制的小鼠的CpG的处理侧（C）的手动标记的每只动物感兴趣区域为两个主要的肿瘤位点。（D）的所述CpG处理或luminoscores之间的比率ODN控制端和PBS控制端是一个索引反映每个肿瘤的相同的动物中的相对增长。这比为1第0天第13天，所述CpG治疗组的比例已经下降显著（P = 0.004），揭示了抑制肿瘤生长在的CpG-DNA 原位管理。 <a href="https://www-jove-com.bdigitaluss.remotexs.co/files/ftp_upload/53609/53609fig3large.jpg" target="_blank">请点击这里查看一个更大的版本这个数字。</a>

Watch this Scientific Journal Video about Bioluminescence-Based Tumor Quantification Method for Monitoring Tumor Progression and Treatment Effects in Mouse Lymphoma Models at JoVE.com

Bioluminescence-Based Tumor Quantification Method for Monitoring Tumor Progression and Treatment Effects in Mouse Lymphoma Models

生物发光成像是用于定位肿瘤和转移一个众所周知的工具，但是这些图像的量化通常需要复杂的计算和特定仪器。我们描述了易于使用luminoscore方法，基于精确采集条件，不需要计算，并且使肿瘤负荷和处理响应于在小鼠模型中进行监测。

对小鼠淋巴瘤模型监测肿瘤进展和治疗效果生物发光基于肿瘤定量方法

Although bioluminescence imaging (BLI) shows promise for monitoring tumor burden in animal models of cancer, these analyses remain mostly qualitative. ...

bioluminescence-based-tumor-quantification-method-for-monitoring

Universidad San Sebastian

Research

JoVE Journal

Medicine

20.1K 次观看.  Genethon. 生物发光成像是用于定位肿瘤和转移一个众所周知的工具，但是这些图像的量化通常需要复杂的计算和特定仪器。我们描述了易于使用luminoscore方法，基于精确采集条件，不需要计算，并且使肿瘤负荷和处理响应于在小鼠模型中进行监测。 

视频: Bioluminescence-Based Tumor Quantification Method for Monitoring Tumor Progression and Treatment Effects in Mouse Lymphoma Models

Murine Bioluminescent Hepatic Tumour Model

This video describes the establishment of liver metastases in a mouse model that can be subsequently analysed by bioluminescent imaging. Tumour cells are administered specifically to the liver to induce a localised liver tumour, via mobilisation of the spleen and splitting into two, leaving intact the vascular pedicle for each half of the spleen. Lewis lung carcinoma cells that constitutively express the firefly luciferase gene (luc1) are inoculated into one hemi-spleen which is then resected 10 minutes later. The other hemi-spleen is left intact and returned to the abdomen. Liver tumour growth can be monitored by bioluminescence imaging using the IVIS whole body imaging system. Quantitative imaging of tumour growth using IVIS provides precise quantitation of viable tumour cells. Tumour cell death and necrosis due to drug treatment is indicated early by a reduction in the bioluminescent signal. This mouse model allows for investigating the mechanisms underlying metastatic tumour-cell survival and growth and can be used for the evaluation of therapeutics of liver metastasis. 

This article describes a procedure for the induction of orthotopic bioluminescent liver tumours in mice, and subsequent analysis of tumour growth confined to the liver using live whole body luminescence imaging.

发光法小鼠肝肿瘤模型

This video describes the establishment of liver metastases in a mouse model that can be subsequently analysed by bioluminescent imaging. Tumour cells are ...

科研

医学

Establishing Intracranial Brain Tumor Xenografts With Subsequent Analysis of Tumor Growth and Response to Therapy using Bioluminescence Imaging

Transplantation models using human brain tumor cells have served an essential function in neuro-oncology research for many years. In the past, the most commonly used procedure for human tumor xenograft establishment consisted of the collection of cells from culture flasks, followed by the subcutaneous injection of the collected cells in immunocompromised mice. Whereas this approach still sees frequent use in many laboratories, there has been a significant shift in emphasis over the past decade towards orthotopic xenograft establishment, which, in the instance of brain tumors, requires tumor cell injection into appropriate neuroanatomical structures. Because intracranial xenograft establishment eliminates the ability to monitor tumor growth through direct measurement, such as by use of calipers, the shift in emphasis towards orthotopic brain tumor xenograft models has necessitated the utilization of non-invasive imaging for assessing tumor burden in host animals. Of the currently available imaging methods, bioluminescence monitoring is generally considered to offer the best combination of sensitivity, expediency, and cost. Here, we will demonstrate procedures for orthotopic brain tumor establishment, and for monitoring tumor growth and response to treatment when testing experimental therapies.

Luciferase-modified human brain tumor xenografts can be established intracranially in athymic mice, with subsequent monitoring of tumor growth and response to therapy using bioluminescence imaging. In combination with survival analysis, bioluminescence monitoring is an essential research tool for pre-clinical testing of therapies being considered for treating brain tumors.

建立颅内脑肿瘤异种移植肿瘤的生长和对治疗的反应与后续利用生物发光成像分析

Transplantation models using human brain tumor cells have served an essential function in neuro-oncology research for many years. In the past, the most ...

神经科学

Orthotopic Xenografting of Human Luciferase-Tagged Malignant Peripheral Nerve Sheath Tumor Cells for in vivo Testing of Candidate Therapeutic Agents

Although in vitro screens are essential for the initial identification of candidate therapeutic agents, a rigorous assessment of the drug's ability to inhibit tumor growth must be performed in a suitable animal model. The type of animal model that is best for this purpose is a topic of intense discussion. Some evidence indicates that preclinical trials examining drug effects on tumors arising in transgenic mice are more predictive of clinical outcome1and so candidate therapeutic agents are often tested in these models. Unfortunately, transgenic models are not available for many tumor types. Further, transgenic models often have other limitations such as concerns as to how well the mouse tumor models its human counterpart, incomplete penetrance of the tumor phenotype and an inability to predict when tumors will develop.
Consequently, many investigators use xenograft models (human tumor cells grafted into immunodeficient mice) for preclinical trials if appropriate transgenic tumor models are not available. Even if transgenic models are available, they are often partnered with xenograft models as the latter facilitate rapid determination of therapeutic ranges. Further, this partnership allows a comparison of the effectiveness of the agent in transgenic tumors and genuine human tumor cells. Historically, xenografting has often been performed by injecting tumor cells subcutaneously (ectopic xenografts). This technique is rapid and reproducible, relatively inexpensive and allows continuous quantitation of tumor growth during the therapeutic period2. However, the subcutaneous space is not the normal microenvironment for most neoplasms and so results obtained with ectopic xenografting can be misleading due to factors such as an absence of organ-specific expression of host tissue and tumor genes. It has thus been strongly recommended that ectopic grafting studies be replaced or complemented by studies in which human tumor cells are grafted into their tissue of origin (orthotopic xenografting)2. Unfortunately, implementation of this recommendation is often thwarted by the fact that orthotopic xenografting methodologies have not yet been developed for many tumor types.
Malignant peripheral nerve sheath tumors (MPNSTs) are highly aggressive sarcomas that occur sporadically or in association with neurofibromatosis type 13and most commonly arise in the sciatic nerve4. Here we describe a technically straightforward method in which firefly luciferase-tagged human MPNST cells are orthopically xenografted into the sciatic nerve of immunodeficient mice. Our approach to assessing the success of the grafting procedure in individual animals and subsequent non-biased randomization into study groups is also discussed.

Orthotopic Xenografting of Human Luciferase-Tagged Malignant Peripheral Nerve Sheath Tumor Cells for in vivo Testing of Candidate Therapeutic Agents

A method for reliably grafting luciferase-tagged human malignant peripheral nerve sheath tumor cells into the sciatic nerve of immunodeficient mice is described. The use of bioluminescence imaging to demonstrate proper establishment of tumor grafts and criteria for random segregation of animals into study groups are also discussed.

原位Xenografting人类荧光素酶标记的恶性外周神经鞘瘤细胞在体内测试候选治疗药物

Although in vitro screens are essential for the initial identification of candidate therapeutic agents, a rigorous assessment of the drug's ability to ...

Monitoring Tumor Metastases and Osteolytic Lesions with Bioluminescence and Micro CT Imaging

Following intracardiac delivery of MDA-MB-231-luc-D3H2LN cells to Nu/Nu mice, systemic metastases developed in the injected animals. Bioluminescence imaging using IVIS Spectrum was employed to monitor the distribution and development of the tumor cells following the delivery procedure including DLIT reconstruction to measure the tumor signal and its location.
Development of metastatic lesions to the bone tissues triggers osteolytic activity and lesions to tibia and femur were evaluated longitudinally using micro CT. Imaging was performed using a Quantum FX micro CT system with fast imaging and low X-ray dose. The low radiation dose allows multiple imaging sessions to be performed with a cumulative X-ray dosage far below LD50. A mouse imaging shuttle device was used to sequentially image the mice with both IVIS Spectrum and Quantum FX achieving accurate animal positioning in both the bioluminescence and CT images. The optical and CT data sets were co-registered in 3-dimentions using the Living Image 4.1 software. This multi-mode approach allows close monitoring of tumor growth and development simultaneously with osteolytic activity.

An experimental mouse model of bone metastasis was established following intracardiac delivery of luciferase expressing mammary tumor cells. Tumor development and resulted osteolytic lesion were monitored longitudinally with bioluminescence and micro CT imaging.

生物发光和微型CT成像监测肿瘤转移和溶骨性病变

Following intracardiac delivery of MDA-MB-231-luc-D3H2LN cells to Nu/Nu mice, systemic metastases developed in the injected animals. Bioluminescence ...

Multimodal Bioluminescent and Positronic-emission Tomography/Computational Tomography Imaging of Multiple Myeloma Bone Marrow Xenografts in NOG Mice

Multiple myeloma (MM) tumors engraft in the bone marrow (BM) and their survival and progression are dependent upon complex molecular and cellular interactions that exist within this microenvironment. Yet the BM microenvironment cannot be easily replicated in vitro, which potentially limits the physiologic relevance of many in vitro and ex vivo experimental models. These issues can be overcome by utilizing a xenograft model in which luciferase (LUC)-transfected 8226 MM cells will specifically engraft in the mouse skeleton. When these mice are given the appropriate substrate, D-luciferin, the effects of therapy on tumor growth and survival can be analyzed by measuring changes in the bioluminescent images (BLI) produced by the tumors in vivo. This BLI data combined with positronic-emission tomography/computational tomography (PET/CT) analysis using the metabolic marker 2-deoxy-2-(18F)fluoro-D-glucose (18F-FDG) is used to monitor changes in tumor metabolism over time. These imaging platforms allow for multiple noninvasive measurements within the tumor/BM microenvironment.

Here we use bioluminescent, X-ray, and positron-emission tomography/computed tomography imaging to study how inhibiting mTOR activity impacts bone marrow-engrafted myeloma tumors in a xenograft model. This allows for physiologically relevant, non-invasive, and multimodal analyses of the anti-myeloma effect of therapies targeting bone marrow-engrafted myeloma tumors in vivo.

nog 小鼠多发性骨髓瘤异种移植的多模态生物发光和正电子发射成形断层扫描成像

Multiple myeloma (MM) tumors engraft in the bone marrow (BM) and their survival and progression are dependent upon complex molecular and cellular ...

癌症研究

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

Triple-negative breast cancer (TNBC) is an aggressive breast cancer subtype with limited therapeutic options. When compared to patients with less aggressive breast tumors, the 5-year survival rate of TNBC patients is 77% due to their characteristic drug-resistant phenotype and metastatic burden. Toward this end, murine models have been established aimed at identifying novel therapeutic strategies limiting TNBC tumor growth and metastatic spread. This work describes a practical guide for the TNBC orthotopic model where MDA-MB-231 breast cancer cells suspended in a basement membrane matrix are implanted in the fourth mammary fat pad, which closely mimics the cancer cell behavior in humans. Measurement of tumors by caliper, lung metastasis assessment via in vivo and ex vivo imaging, and molecular detection are discussed. This model provides an excellent platform to study therapeutic efficacy and is especially suitable for the study of the interaction between the primary tumor and distal metastatic sites.

This work presets an advanced protocol to accurately assess tumor loading by detection of green fluorescent protein and bioluminescence signals as well as the integration of quantitative molecular detection technique.

研究使用正交乳腺癌模型的三阴性乳腺癌

Triple-negative breast cancer (TNBC) is an aggressive breast cancer subtype with limited therapeutic options. When compared to patients with less ...

Monitoring Breast Cancer Growth and Metastatic Colony Formation in Mice using Bioluminescence

Breast cancer is a frequent heterogeneous malignancy and the second leading cause of mortality in women, mainly due to distant organ metastasis. Several animal models have been generated, including the widely used orthotopic mouse models, where cancer cells are injected into the mammary fat pad. However, these models cannot help monitor tumor growth kinetics and metastatic colonization. Cutting-edge tools to monitor cancer cells in real time in mice will significantly advance the understanding of tumor biology. 
Here, breast cancer cell lines stably expressing luciferase and green fluorescent protein (GFP) were established. Specifically, this technique contains two sequential steps initiated by measuring the luciferase activity in vitro and followed by the implantation of the cancer cells into mammary fat pads of nonobese diabetic-severe combined immunodeficiency (NOD-SCID) mice. After the injection, both the tumor growth and metastatic colonization are monitored in real time by the noninvasive bioluminescence imaging system. Then, the quantification of GFP-expressing metastases in the lungs will be examined by fluorescence microscopy to validate the observed bioluminescence results. This sophisticated system combining luciferase and fluorescence-based detection tools evaluates cancer metastasis in vivo, which has great potential for use in breast cancer therapeutics and disease management.

Here, we describe a noninvasive monitoring method involving luciferase and green fluorescent protein expression in various breast cancer cell lines. This protocol provides a technique to monitor tumor formation and metastatic colonization in real time in mice.

使用生物发光监测小鼠乳腺癌生长和转移集落形成

Breast cancer is a frequent heterogeneous malignancy and the second leading cause of mortality in women, mainly due to distant organ metastasis. Several ...

Bioluminescence Imaging of an Immunocompetent Animal Model for Glioblastoma

In contrast to commonly reported human glioma xenograft animal models, GL261 murine glioma xenografts recapitulate nearly all relevant clinical and histopathologic features of the human disease. When GL261 cells are implanted intracranially in syngeneic C57BL/6 mice, the model has the added advantage of maintaining an intact immune microenvironment. Stable expression of luciferase in GL261 cells allows non-invasive cost effective bioluminescence monitoring of intracranial tumor growth. We have recently demonstrated that luciferase expression in GL261 cells does not affect the tumor growth properties, tumor cell immunomodulatory cytokine expression, infiltration of immune cells into the tumor, or overall survival of animals bearing the intracranial tumor. Therefore, it appears that the GL261 luciferase glioma model can be useful in the study of novel chemotherapeutic and immunotherapeutic modalities. Here we report the technique for generating stable luciferase expression in GL261 cells and how to study the in vitro and in vivo growth of the tumor cells by bioluminescence imaging.

GL261 glioma cells provide a useful immunocompetent animal model of glioblastoma. The goals of this protocol are to demonstrate proper techniques for monitoring intracranial tumor growth using in vivo bioluminescence imaging, and to verify the utility of luciferase-modified GL261 cells for studying tumor immunology and immunotherapeutic approaches for treating glioblastoma.

免疫活性动物模型胶质母细胞瘤的生物发光成像

In contrast to commonly reported human glioma xenograft animal models, GL261 murine glioma xenografts recapitulate nearly all relevant clinical and ...

Generating a Murine Orthotopic Metastatic Breast Cancer Model and Performing Murine Radical Mastectomy

In vivo mouse models to assess breast cancer progression are essential for cancer research, including preclinical drug developments. However, the majority of the practical and technical details are commonly omitted in published manuscripts which, therefore, makes it challenging to reproduce the models, particularly when it involves surgical techniques. Bioluminescence technology allows for the evaluation of small amounts of cancer cells even when a tumor is not palpable. Utilizing luciferase-expressing cancer cells, we establish a breast cancer orthotopic inoculation technique with a high tumorigenesis rate. Lung metastasis is assessed utilizing an ex vivo technique. We, then, establish a mastectomy model with a low local recurrence rate to assess the metastatic tumor burden. Herein, we describe, in detail, the surgical techniques of orthotopic implantation and mastectomy for breast cancer with a high tumorigenesis rate and low local recurrence rates, respectively, to improve breast cancer model efficiency.

We introduce a murine orthotopic breast cancer model and radical mastectomy model with bioluminescence technology to quantify the tumor burden to mimic human breast cancer progression.

小鼠原位转移性乳腺癌模型的建立及小鼠根治性乳房切除术

In vivo mouse models to assess breast cancer progression are essential for cancer research, including preclinical drug developments. However, the majority ...

Dual Bioluminescence Imaging of Tumor Progression and Angiogenesis

Angiogenesis, as a crucial process of tumor progression, has become a research hotspot and target of anti-tumor therapy. However, there is no reliable model for tracing tumor progression and angiogenesis simultaneously in a visual and sensitive manner. Bioluminescence imaging displays its unique superiority in living imaging due to its advantages of high sensitivity, strong specificity, and accurate measurement. Presented here is a protocol to establish a tumor-bearing mouse model by injecting a Renilla luciferase-labeled murine breast cancer cell line 4T1 into the transgenic mouse with angiogenesis-induced Firefly luciferase expression. This mouse model provides a valuable tool to simultaneously monitor tumor progression and angiogenesis in real-time by dual bioluminescence imaging in a single mouse. This model may be widely applied in anti-tumor drug screening and oncology research.

This protocol describes the establishment of a tumor-bearing mouse model to monitor tumor progression and angiogenesis in real-time by dual bioluminescence imaging.

肿瘤进展和血管生成的双生物发光成像

Angiogenesis, as a crucial process of tumor progression, has become a research hotspot and target of anti-tumor therapy. However, there is no reliable ...