神经细胞类型的流式细胞仪协议的表面和细胞内抗原分析

1:03

Neural Cell Harvesting

3:10

Antibody-independent Intracellular Labeling with Carboxyfluorescein Succinimidyl Ester (CFSE)

4:49

Cell-surface Antigen Staining with Fluorophore-conjugated Antibodies

5:56

Fixation and Membrane Permeabilization

7:35

Intracellular Antigen Staining with Non-fluorescent Primary Antibodies

9:30

Flow Cytometry Analysis

10:37

Results: Multicolor Flow Cytometric Analysis of Surface vs. Introcellular Antigens in Neural Cells

12:36

Conclusion